LifeWatch ERIC Metadata Catalogue
The InBIO Barcoding Initiative Database: Diptera 01
The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Default
Identification
- Alternate Identifier
- 10.15468/q1bvt3
- Alternate Identifier
- 21feb87f-62f2-4765-8aa6-6953ee5e09d6
- Alternate Identifier
- http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_i
- Publication Date
- 2021-02-09
- Title
- The InBIO Barcoding Initiative Database: Diptera 01
- Abstract
- The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
- purpose
- The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
- Dataset Language
- ENGLISH
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Creator
- Dataset Creator
-
Computational Biology and Population Genomics Group, cE3c, Faculdade de Ciências, Universidade de Lisboa - Ana Rita Gonçalves (Master student )
Campo Grande , Lisboa , Lisboa , 1749-016 , PORTUGAL
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
- Metadata Provider
-
Computational Biology and Population Genomics Group, cE3c, Faculdade de Ciências, Universidade de Lisboa - Ana Rita Gonçalves (Master student )
Campo Grande , Lisboa , Lisboa , 1749-016 , PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
-
- Diptera
- Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
-
- Species
- Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
-
- Occurrence
- Keywords (n/a )
-
- Cytochrome c oxidase I (COI)
- Keywords (n/a )
-
- DNA barcode
- Keywords (n/a )
-
- Continental Portugal
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
-
- Occurrence
Geographic Coverage
- Geographic Description
- Continental Portugal
Bounding Box
- West Bounding Coordinate
- -8.774
- East Bounding Coordinate
- -6.274
- North Bounding Coordinate
- 42.002
- South Bounding Coordinate
- 40.054
Temporal Coverage
Range of Dates
- Begin Date
-
2014-05-27
- End Date
-
2018-07-07
Taxonomic Coverage
- General Taxonomic Coverage
- This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.
Taxonomic Classification
- Taxonomic Rank Name
- Kingdom
- Taxonomic Rank Value
- Animalia
Taxonomic Classification
- Taxonomic Rank Name
- Phylum
- Taxonomic Rank Value
- Arthropoda
Taxonomic Classification
- Taxonomic Rank Name
- Class
- Taxonomic Rank Value
- Insecta
Taxonomic Classification
- Taxonomic Rank Name
- Order
- Taxonomic Rank Value
- Diptera
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Anisopodidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Anthomyiidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Asilidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Bibionidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Bombyliidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Calliphoridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Canacidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Chloropidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Conopidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Diastatidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Dolichopodidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Drosophilidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Ephydridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Fanniidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Heleomyzidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Hippoboscidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Hybotidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Lauxaniidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Lonchaeidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Micropezidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Muscidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Nemestrinidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Opetiidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Opomyzidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Pallopteridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Periscelididae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Platystomatidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Psychodidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Ptychopteridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Rhiniidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Rhinophoridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Sarcophagidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Scathophagidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Sciomyzidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Stratiomyidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Syrphidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Tabanidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Tachinidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Tephritidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Trichoceridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Ulidiidae
License Information
- Intellectual Rights
-
This work is licensed under a
Creative Commons Attribution (CC-BY) 4.0 License
.
Distributiondocument
Projects
Projects
• ProjectInBIO Barcoding Initiative
- Title
- The InBIO Barcoding Initiative Database: Diptera 01
Personnel
Individual Name
- Given Name
- Sónia
- Surname
- Ferreira
- User ID
- 0000-0002-6884-3966
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Rui
- Surname
- Andrade
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Ana Rita
- Surname
- Gonçalves
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Sousa
- User ID
- 0000-0002-5859-9656
- Role
- ORIGINATOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Beja
- User ID
- 0000-0001-8164-0760
- Role
- AUTHOR
- Abstract
- The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
- Funding
- This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC).
Study Area Description
- Descriptor
-
Specimens were collected in eight continental Portugal districts. Fifty-nine different localities were sampled encompassing a great variety of habitats. Sampling was conducted from 2014 to 2018, with more than half of the specimens (62.6%) collected in the year 2017.
Design Description
- Description
- Specimens in this dataset were collected in field work carried out between 2014 and 2018. Specimens resulted from field work directed specifically for the sampling of Diptera using different methodologies.
Methods
Methods
• Method
Method Step
- Description
- Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
Method Step
- Description
- Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
Method Step
- Description
- Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
Method Step
- Description
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
Method Step
- Description
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
Method Step
- Description
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Sampling
- Study Extent
-
Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017.
- Sampling Description
- The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)
qualityControl
- Description
- To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.
