The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification

The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Default

Identification

Alternate Identifier
10.15468/x7yk7m
Alternate Identifier
22f83f75-3b92-4db6-a86e-8dac512c4e65
Alternate Identifier
http://ipt.gbif.pt/ipt/resource?r=ibi_megaloptera_sialis
Publication Date
2021-01-05
Title
The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification
Abstract
The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Dataset Language
ENGLISH
 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Joana Paupério (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  Museu de História Natural e da Ciência da Universidade do Porto - José Manuel Grosso-Silva (Entomology Curator )
Praça Gomes Teixeira , Porto , Porto , 4099-002 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Joana Paupério (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  Museu de História Natural e da Ciência da Universidade do Porto - José Manuel Grosso-Silva (Entomology Curator )
Praça Gomes Teixeira , Porto , Porto , 4099-002 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Keywords (n/a )
  • Megaloptera
Keywords (n/a )
  • Sialidae
Keywords (n/a )
  • Species
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
  • Occurrence
Keywords (n/a )
  • Cytochrome c oxidase I (COI)
Keywords (n/a )
  • DNA barcode
Keywords (n/a )
  • Continental Portugal
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
  • Occurrence

Geographic Coverage

Geographic Description
Continental Portugal.

Bounding Box

West Bounding Coordinate
-7.011
East Bounding Coordinate
-6.556
North Bounding Coordinate
41.775
South Bounding Coordinate
41.527

Temporal Coverage

Range of Dates

Begin Date

2011-06-27

End Date

2015-04-30

Taxonomic Coverage

General Taxonomic Coverage
This dataset is composed of data relating to five Megaloptera specimens. All specimens were determined to species level. All specimens belong to the genus Sialis Latreille, 1803, and the family Sialidae.

Taxonomic Classification

Taxonomic Rank Name
Kingdom
Taxonomic Rank Value
Animalia

Taxonomic Classification

Taxonomic Rank Name
Phylum
Taxonomic Rank Value
Arthropoda

Taxonomic Classification

Taxonomic Rank Name
Class
Taxonomic Rank Value
Insecta

Taxonomic Classification

Taxonomic Rank Name
Order
Taxonomic Rank Value
Megaloptera

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Sialidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 

Distributiondocument

Online

URL
http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IBIMP1
 
 

Projects

Projects

• ProjectInBIO Barcoding Initiative

Title
The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification

Personnel

Individual Name

Given Name
Pedro
Surname
Beja
User ID
0000-0001-8164-0760
Role
AUTHOR

Personnel

Individual Name

Given Name
Sónia
Surname
Ferreira
User ID
0000-0002-6884-3966
Role
AUTHOR

Personnel

Individual Name

Given Name
Joana
Surname
Paupério
User ID
0000-0003-2569-0768
Role
AUTHOR

Personnel

Individual Name

Given Name
José Manuel
Surname
Grosso-Silva
User ID
0000-0002-1094-0771
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro
Surname
Sousa
User ID
0000-0002-5859-9656
Role
ORIGINATOR
Abstract
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
Funding
InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).

Study Area Description

Descriptor

Specimens were collected in continental Portugal, in four different localities. Sampling was conducted in 2011 and 2015.

Design Description

Description
Specimens in this dataset were collected in field work carried in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.
 
 

Methods

Methods

• Method

Method Step

Description
Sampling of specimens was done at four different localities of continental Portugal, in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.

Method Step

Description
The captured specimens were preserved in 96% ethanol.

Method Step

Description
The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Monserrat, 2014).

Method Step

Description
DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.

Method Step

Description
The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.

Method Step

Description
Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in four different localities of continental Portugal. Sampling was conducted in 2011 and 2015.

Sampling Description
Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

qualityControl

Description
The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
 
 

Not available