The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)

The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal

Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

Default

Identification

Alternate Identifier
10.15468/rmj9sz
Alternate Identifier
7b84fe1d-42d0-439a-b60f-0b621f7ba93d
Alternate Identifier
http://ipt.gbif.pt/ipt/resource?r=ibi_mecoptera
Publication Date
2020-08-03
Title
The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)
Abstract

The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal

Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Dataset Language
ENGLISH
 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Daniel Oliveira (Master’s Student )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Brito Lopes (Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Daniel Oliveira (Master’s Student )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Brito Lopes (Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Keywords (n/a )
  • Mecoptera
Keywords (n/a )
  • Panorpidae
Keywords (n/a )
  • Species
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
  • Occurrence
Keywords (n/a )
  • Cytochrome c oxidase I (COI)
Keywords (n/a )
  • DNA barcode
Keywords (n/a )
  • Continental Portugal
Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
  • Specimen

Geographic Coverage

Geographic Description
Continental Portugal

Bounding Box

West Bounding Coordinate
-8.7
East Bounding Coordinate
-6.4
North Bounding Coordinate
42
South Bounding Coordinate
39.8

Temporal Coverage

Range of Dates

Begin Date

2008-06-02

End Date

2019-06-28

Taxonomic Coverage

General Taxonomic Coverage
This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal.

Taxonomic Classification

Taxonomic Rank Name
Kingdom
Taxonomic Rank Value
Animalia

Taxonomic Classification

Taxonomic Rank Name
Phylum
Taxonomic Rank Value
Arthropoda

Taxonomic Classification

Taxonomic Rank Name
Class
Taxonomic Rank Value
Insecta

Taxonomic Classification

Taxonomic Rank Name
Order
Taxonomic Rank Value
Mecoptera

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Panorpidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 

Distributiondocument

Online

URL
http://www.boldsystems.org
 
 

Projects

Projects

• ProjectInBIO Barcoding Initiative

Title
The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)

Personnel

Individual Name

Given Name
Pedro
Surname
Beja
User ID
0000-0001-8164-0760
Role
AUTHOR

Personnel

Individual Name

Given Name
Daniel
Surname
Oliveira
User ID
0000-0002-8733-4104
Role
AUTHOR

Personnel

Individual Name

Given Name
Luís
Surname
P. da Silva
User ID
0000-0003-2358-1277
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro Brito
Surname
Lopes
User ID
0000-0002-5078-0602
Role
AUTHOR

Personnel

Individual Name

Given Name
Sónia
Surname
Ferreira
User ID
0000-0002-6884-3966
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro
Surname
Sousa
User ID
0000-0002-5859-9656
Role
ORIGINATOR
Abstract
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
Funding
InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250.

Study Area Description

Descriptor

Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts.

Design Description

Description
The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search.
 
 

Methods

Methods

• Method

Method Step

Description
Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.

Method Step

Description
The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013).

Method Step

Description
DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.

Method Step

Description
All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.

Method Step

Description
Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions.

Sampling Description
Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.

qualityControl

Description
To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
 
 

Not available