LifeWatch ERIC Metadata Catalogue
The InBIO Barcoding Initiative Database: Hemiptera 01
The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Default
Identification
- Alternate Identifier
- 10.15468/8hn25y
- Alternate Identifier
- 963a25a6-839c-4bff-9889-97fc8385f564
- Alternate Identifier
- http://ipt.gbif.pt/ipt/resource?r=ibi_hemiptera_01
- Publication Date
- 2021-07-30
- Title
- The InBIO Barcoding Initiative Database: Hemiptera 01
- Abstract
- The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
- purpose
- The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
- Dataset Language
- ENGLISH
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Creator
- Dataset Creator
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
- Metadata Provider
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
-
- Hemiptera
- Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
-
- Species
- Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
-
- Occurrence
- Keywords (n/a )
-
- Cytochrome c oxidase I (COI)
- Keywords (n/a )
-
- DNA barcode
- Keywords (n/a )
-
- Continental Portugal
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
-
- Occurrence
Geographic Coverage
- Geographic Description
- Continental Portugal.
Bounding Box
- West Bounding Coordinate
- -9.465
- East Bounding Coordinate
- -6.344
- North Bounding Coordinate
- 41.979
- South Bounding Coordinate
- 37.257
Temporal Coverage
Range of Dates
- Begin Date
-
2015-03-16
- End Date
-
2019-09-20
Taxonomic Coverage
- General Taxonomic Coverage
- This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.
Taxonomic Classification
- Taxonomic Rank Name
- Kingdom
- Taxonomic Rank Value
- Animalia
Taxonomic Classification
- Taxonomic Rank Name
- Phylum
- Taxonomic Rank Value
- Arthropoda
Taxonomic Classification
- Taxonomic Rank Name
- Class
- Taxonomic Rank Value
- Insecta
Taxonomic Classification
- Taxonomic Rank Name
- Order
- Taxonomic Rank Value
- Hemiptera
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Acanthosomatidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Alydidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Aphalaridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Aphrophoridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Aradidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Auchenorrhyncha
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Berytidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Cercopidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Cicadellidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Cicadidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Coreidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Corixidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Cydnidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Delphacidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Dictyopharidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Flatidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Gerridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Heteroptera
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Hydrometridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Lygaeidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Membracidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Miridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Nabidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Nepidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Notonectidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Pentatomidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Potamocoridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Pyrrhocoridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Reduviidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Rhopalidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Rhyparochromidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Stenocephalidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Thaumastocoridae
License Information
- Intellectual Rights
-
This work is licensed under a
Creative Commons Attribution (CC-BY) 4.0 License
.
Distributiondocument
Projects
Projects
• ProjectInBIO Barcoding Initiative
- Title
- The InBIO Barcoding Initiative Database: Hemiptera 01
Personnel
Individual Name
- Given Name
- Sónia
- Surname
- Ferreira
- User ID
- 0000-0002-6884-3966
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- José M.
- Surname
- Grosso-Silva
- User ID
- 0000-0002-1094-0771
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Rui
- Surname
- Andrade
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Sousa
- User ID
- 0000-0002-5859-9656
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Beja
- User ID
- 0000-0001-8164-0760
- Role
- AUTHOR
- Abstract
- The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
- Funding
- This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC).
Study Area Description
- Descriptor
-
Specimens were collected in 12 continental Portugal districts. Sixty different localities were, almost half of which (47%) from the Bragança district. Sampling was conducted from 2015 to 2019, with 39% of the specimens collected in the year 2018.
Design Description
- Description
- Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens resulted from fieldwork collection by direct search of specimens or by sweeping the vegetation.
Methods
Methods
• Method
Method Step
- Description
- Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
Method Step
- Description
- Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
Method Step
- Description
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
Method Step
- Description
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
Method Step
- Description
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
Method Step
- Description
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Sampling
- Study Extent
-
Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.
- Sampling Description
- The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.
qualityControl
- Description
- All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).
