The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)

The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Identification

Alternate Identifier: 10.15468/9z8bpm

Alternate Identifier: cd11c12e-1f1c-4ec6-8b28-c57425956915

Alternate Identifier: http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana

Publication Date: 2021-01-05

Title: The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)

Abstract: The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Dataset Language: ENGLISH

Dataset Creator: Martin Corley (Researcher )

Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: Martin Corley (Researcher )

Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Keywords (n/a )

Lepidoptera

Keywords (n/a )

Species

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )

Occurrence

Keywords (n/a )

Cytochrome c oxidase I (COI)

Keywords (n/a )

DNA barcode

Keywords (n/a )

Continental Portugal

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )

Occurrence

Geographic Coverage

Geographic Description: Continental Portugal.

Bounding Box

West Bounding Coordinate: -8.169

East Bounding Coordinate: -7.004

North Bounding Coordinate: 42.003

South Bounding Coordinate: 41.422

Temporal Coverage

Range of Dates

Begin Date

2012-06-14

End Date

2016-05-31

Taxonomic Coverage

General Taxonomic Coverage: This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.

Taxonomic Classification

Taxonomic Rank Name: Kingdom

Taxonomic Rank Value: Animalia

Taxonomic Classification

Taxonomic Rank Name: Phylum

Taxonomic Rank Value: Arthropoda

Taxonomic Classification

Taxonomic Rank Name: Class

Taxonomic Rank Value: Insecta

Taxonomic Classification

Taxonomic Rank Name: Order

Taxonomic Rank Value: Lepidoptera

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Tortricidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

Distributiondocument

Online

URL: http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IBILP6

Projects

• ProjectInBIO Barcoding Initiative

Title: The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)

Personnel

Individual Name

Given Name: Pedro

Surname: Beja

User ID: 0000-0001-8164-0760

Role: AUTHOR

Personnel

Individual Name

Given Name: Martin

Surname: Corley

User ID: 0000-0003-4240-8007

Role: AUTHOR

Personnel

Individual Name

Given Name: Sónia

Surname: Ferreira

User ID: 0000-0002-6884-3966

Role: AUTHOR

Personnel

Individual Name

Given Name: Pedro

Surname: Sousa

User ID: 0000-0002-5859-9656

Role: ORIGINATOR

Abstract: The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Funding: InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).

Study Area Description

Descriptor

Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016.

Design Description

Description: Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night.

Methods

• Method

Method Step

Description: Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.

Method Step

Description: The captured specimens were preserved in dry storage.

Method Step

Description: The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).

Method Step

Description: DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.

Method Step

Description: The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.

Method Step

Description: Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016.

Sampling Description: Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

qualityControl

Description: The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Not available

Default

Identification

Geographic Coverage

Bounding Box

Temporal Coverage

Range of Dates

Taxonomic Coverage

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

License Information

Distributiondocument

Online

Projects

Projects

• ProjectInBIO Barcoding Initiative

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Study Area Description

Design Description

Methods

Methods

• Method

Method Step

Method Step

Method Step

Method Step

Method Step

Method Step

Sampling

qualityControl