The InBIO Barcoding Initiative Database: Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group

The dataset contains two records of moth specimens (Lepidoptera, Depressariidae) collected in 2011 and 2015 in continental Portugal. The study of these specimens resulted in the description of a new Portuguese endemic species, Depressaria infernella Corley & Buchner, 2019 based on differences found in the male and female genitalia and DNA barcodes in Corley et al. (2019). Specimens were detected and captured using a mercury-vapor lamp to attract the insects. Captured specimens were identified to species level and preserved in 96% ethanol or in dry storage. From each specimen a tissue sample, one leg, was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Collected specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Identification

Alternate Identifier: 10.15468/gcg7ny

Alternate Identifier: f90de717-7b3f-483f-aca2-46c300fe36a8

Alternate Identifier: http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_depressaria_infernella

Publication Date: 2021-01-05

Title: The InBIO Barcoding Initiative Database: Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group

Abstract: The dataset contains two records of moth specimens (Lepidoptera, Depressariidae) collected in 2011 and 2015 in continental Portugal. The study of these specimens resulted in the description of a new Portuguese endemic species, Depressaria infernella Corley & Buchner, 2019 based on differences found in the male and female genitalia and DNA barcodes in Corley et al. (2019). Specimens were detected and captured using a mercury-vapor lamp to attract the insects. Captured specimens were identified to species level and preserved in 96% ethanol or in dry storage. From each specimen a tissue sample, one leg, was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Collected specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Dataset Language: ENGLISH

Dataset Creator: Martin Corley (Researcher )

Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Creator: Peter Buchner (Researcher )

Schwarzau am Steinfeld , A-2625 , AUSTRIA

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: Martin Corley (Researcher )

Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: Peter Buchner (Researcher )

Schwarzau am Steinfeld , A-2625 , AUSTRIA

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL

Keywords (n/a )

Lepidoptera

Keywords (n/a )

Species

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )

Occurrence

Keywords (n/a )

Cytochrome c oxidase I (COI)

Keywords (n/a )

DNA barcode

Keywords (n/a )

Continental Portugal

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )

Occurrence

Geographic Coverage

Geographic Description: Continental Portugal.

Bounding Box

West Bounding Coordinate: -7.573

East Bounding Coordinate: -6.902

North Bounding Coordinate: 41.766

South Bounding Coordinate: 40.326

Temporal Coverage

Range of Dates

Begin Date

2011-08-05

End Date

2015-06-19

Taxonomic Coverage

General Taxonomic Coverage: This dataset is composed of data relating to two Lepidoptera specimens. Both specimens were determined to species level. The dataset is composed of two species, Depressaria infernella Corley & Buchner, 2019 and Agonopterix scopariella (Heinemann, 1870). Both species belong to the family Depressariidae.

Taxonomic Classification

Taxonomic Rank Name: Kingdom

Taxonomic Rank Value: Animalia

Taxonomic Classification

Taxonomic Rank Name: Phylum

Taxonomic Rank Value: Arthropoda

Taxonomic Classification

Taxonomic Rank Name: Class

Taxonomic Rank Value: Insecta

Taxonomic Classification

Taxonomic Rank Name: Order

Taxonomic Rank Value: Lepidoptera

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Depressariidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

Distributiondocument

Online

URL: http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IBILP3

Projects

• ProjectInBIO Barcoding Initiative

Title: The InBIO Barcoding Initiative Database: Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group

Personnel

Individual Name

Given Name: Pedro

Surname: Beja

User ID: 0000-0001-8164-0760

Role: AUTHOR

Personnel

Individual Name

Given Name: Sónia

Surname: Ferreira

User ID: 0000-0002-6884-3966

Role: AUTHOR

Personnel

Individual Name

Given Name: Martin

Surname: Corley

User ID: 0000-0003-4240-8007

Role: AUTHOR

Personnel

Individual Name

Given Name: Peter

Surname: Buchner

Role: AUTHOR

Personnel

Individual Name

Given Name: Pedro

Surname: Sousa

User ID: 0000-0002-5859-9656

Role: ORIGINATOR

Abstract: The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Funding: InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).

Study Area Description

Descriptor

The two specimens were collected in the districts of Bragança and Guarda, in Continental Portugal, in 2011 and 2015.

Design Description

Description: The specimens in this dataset were collected in Continental Portugal in two field work incursions carried out in 2011 and 2015. Specimens were detected using a mercury-vapor lamps as lures.

Methods

• Method

Method Step

Description: Specimens were captured in two localities of continental Portugal, in 2011 and 2015, using mercury-vapor lamps as lures.

Method Step

Description: Captured specimens were preserved in 96% ethanol or in dry storage.

Method Step

Description: The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley, Buchner & Ferreira, 2019; Hannemann, 1953; Palm, 1989.)

Method Step

Description: A DNA barcode was sequenced from a tissue sample, one leg, that was removed. DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced.

Method Step

Description: The obtained sequences were submitted to BOLD database and the specimen morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.

Method Step

Description: Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in Continental Portugal in two field work incursions carried out in 2011 and 2015.

Sampling Description: Specimens were captured in 2011 and 2015 in continental Portugal uisng mercury-vapor lamps as lures. Captured insects were preserved in 96% ethanol or in dry storage and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.

qualityControl

Description: The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Not available

Default

Identification

Geographic Coverage

Bounding Box

Temporal Coverage

Range of Dates

Taxonomic Coverage

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

License Information

Distributiondocument

Online

Projects

Projects

• ProjectInBIO Barcoding Initiative

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Study Area Description

Design Description

Methods

Methods

• Method

Method Step

Method Step

Method Step

Method Step

Method Step

Method Step

Sampling

qualityControl