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    The dataset contains records of bats (Chiroptera) collected in the lower valley of River Tua. Data were collected in the scope of the Complementary Studies (2010-2011) of the Environmental Impact Assessment of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected in April 2010 during field visits to 48 points located in suitable foraging habitats for bats, within a 5-km radius buffer around the lower valley of River Tua. The presence and identity of the bat species was assessed by acoustic sampling of the echo-location calls emitted in fligth. The records include taxa identified at the species or genus level, as well as groups of species corresponding to seven acoustic “species complexes”.