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    The dataset contains records of nocturnal birds (Aves: Strigidae), and particularly the Eagle Owl (Bubo bubo) collected in the lower valley of the River Tua and included in the Ecological Monitoring Program (2011-2017) of the Environmental Impact Assessment of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected between January 2011 and June 2017 during field visits to 22 sampling points on the area of the lower Tua valley directly affected by the Foz-Tua dam. The presence of individuals was recorded by acoustic observation.