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This is one of the three input files required for running Step 1 (Metabarcoding Runner) of the Metabarcoding workflow. This file in Darwin Core format (https://dwc.tdwg.org/terms/) contains eDNA sample collection information including the location and the geographical coordinates of the eDNA sampling sites. The other two input files for Step 1 are the "Fastq" file with sequences generated from High Throughput Sequencing of eDNA samples and the "Reference_db" with reference sequences for the taxonomic assignment of eDNA sequences.