The dataset includes 360 records with 236 carbon and nitrogen isotopic signatures of Callinectes sapidus and 224 isotopic data of potential prey gathered from five countries and 12 locations between 2014 and 2019.

Two geo-referenced datasets with carbon and nitrogen isotopic values of Procambarus clarkii in invaded inland waters resolved respectively at population- and individual-scale. The population-scale dataset consists of 160 δ13C and δ15N isotopic values of P. clarkii and its potential prey, including living and non-living primary producers and benthic invertebrates. The individual-scale dataset consists of 1,168 isotopic individual records of P. clarkii. The isotopic information included within the two datasets was gathered from 10 countries located in Europe, Asia, Africa, and North America, and from a total of 41 studies published between 2005 and 2021.