The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera

The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Identification

Alternate Identifier: 50942caf-a62e-44c7-9998-e2a949aa85b2

Publication Date: 2021-02-09

Title: The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera

Abstract: The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Dataset Language: ENGLISH

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

PORTUGAL

Dataset Creator: Universidad de Granada - J. Manuel Tierno de Figueroa (Full Professor)

SPAIN

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Filipa M S Martins (PhD student)

PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Joana Veríssimo (PhD student)

PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Lorenzo Quaglietta (Post-Doc Researcher)

PORTUGAL

Dataset Creator: Museu de História Natural e da Ciência da Universidade do Porto - José M. Grosso-Silva (Entomology Curator)

PORTUGAL

Dataset Creator: Pedro Brito Lopes (Researcher)

Rua do Torgal, nº 16 (Trigais)
,
Covilhã
,
PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

PORTUGAL

Dataset Creator: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

PORTUGAL

Metadata Provider: Universidad de Granada - J. Manuel Tierno de Figueroa (Full Professor)

SPAIN

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Filipa M S Martins (PhD student)

PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Joana Veríssimo (PhD student)

PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Lorenzo Quaglietta (Post-Doc Researcher)

PORTUGAL

Metadata Provider: Museu de História Natural e da Ciência da Universidade do Porto - José M. Grosso-Silva (Entomology Curator)

PORTUGAL

Metadata Provider: Pedro Brito Lopes (Researcher)

Rua do Torgal, nº 16 (Trigais)
,
Covilhã
,
PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

PORTUGAL

Metadata Provider: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

PORTUGAL

Dataset Contact: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

PORTUGAL

Keywords (n/a)

Plecoptera

Keywords (n/a)

Occurrence records

Keywords (n/a)

Species distributions

Keywords (n/a)

Continental Portugal

Keywords (n/a)

Continental Spain

Keywords (n/a)

DNA barcode

Keywords (n/a)

Cytochrome c oxidase I (COI)

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml)

Occurrence

Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml)

Specimen

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml)

Occurrence

Geographic Coverage

Geographic Description: Iberian Peninsula

Bounding Box

West Bounding Coordinate: -7.749

East Bounding Coordinate: 3.036

North Bounding Coordinate: 42.506

South Bounding Coordinate: 36.617

Temporal Coverage

Range of Dates

Begin Date: 2004-06-22

End Date: 2018-05-19

Taxonomic Coverage

General Taxonomic Coverage: This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.

Taxonomic Classification

Taxonomic Rank Name: Kingdom

Taxonomic Rank Value: Animalia

Taxonomic Classification

Taxonomic Rank Name: Phylum

Taxonomic Rank Value: Arthropoda

Taxonomic Classification

Taxonomic Rank Name: Class

Taxonomic Rank Value: Insecta

Taxonomic Classification

Taxonomic Rank Name: Order

Taxonomic Rank Value: Plecoptera

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Capniidae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Chloroperlidae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Leuctridae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Nemouridae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Perlidae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Perlodidae

Taxonomic Classification

Taxonomic Rank Name: Family

Taxonomic Rank Value: Taeniopterygidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

Distribution

Online

URL: http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IBIPP01

Additional Metadata

Metadata

GBIF Metadata Block

Date Stamp: 2021-11-29T09:05:31Z

Citation: Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2021). The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Version 1.6. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/3vkdr2 accessed via GBIF.org on 2021-11-29.

Bibliography

Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp.

Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=Jidtax=571. Accessed on: 2020-4-09.

Ferreira, S., Tierno de Figueroa, J.M., Martins, F.M., Verissimo, J., Quaglietta, L., Grosso-Silva, J.M., Lopes, P.B., Sousa, P., Paupério, J., Fonseca, N.A. Beja, P. (2020). The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Biodiversity Data Journal, 8: e55137. https://doi.org/10.3897/BDJ.8.e55137

Collection

4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970

InBIO Barcoding Initiative

Speciment Preservation Method: ALCOHOL

JGTI Curatorial Unit

Voucher tube

JGTI Curatorial Unit

DNA extractions

• Project

Title: The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera

Personnel

Individual Name

Given Name: Sónia

Surname: Ferreira

User ID: 0000-0002-6884-3966

Role: AUTHOR

Personnel

Individual Name

Given Name: J. Manuel

Surname: Tierno de Figueroa

User ID: 0000-0003-1616-9815

Role: AUTHOR

Personnel

Individual Name

Given Name: Filipa

Surname: Martins

User ID: 0000-0003-4191-5031

Role: AUTHOR

Personnel

Individual Name

Given Name: Joana

Surname: Veríssimo

User ID: 0000-0002-8721-9376

Role: AUTHOR

Personnel

Individual Name

Given Name: Lorenzo

Surname: Quaglietta

User ID: 0000-0002-9703-0171

Role: AUTHOR

Personnel

Individual Name

Given Name: José M.

Surname: Grosso-Silva

User ID: 0000-0002-1094-0771

Role: AUTHOR

Personnel

Individual Name

Given Name: Pedro Brito

Surname: Lopes

User ID: 0000-0002-5078-0602

Role: AUTHOR

Personnel

Individual Name

Given Name: Pedro

Surname: Sousa

User ID: 0000-0002-5859-9656

Role: ORIGINATOR

Personnel

Individual Name

Given Name: Pedro

Surname: Beja

User ID: 0000-0001-8164-0760

Role: AUTHOR

Abstract: The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Funding: This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017.

Study Area Description

Descriptor: Iberian Peninsula.

Design Description

Description: Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded.

• Method

Method Step

Description: Specimens were collected in 40 different localities of the Iberian Peninsula. Fieldwork was carried out from 2004 to 2018.

Method Step

Description: Specimens were collected during fieldwork by direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Collected specimens were stored in 96% ethanol.

Method Step

Description: Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature.

Method Step

Description: DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).

Method Step

Description: All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.

Method Step

Description: Data was submitted to GBIF, after being checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org/).

Sampling

Study Extent: Iberian Peninsula.

Sampling Description: The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).

qualityControl

Description: All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 ( http://openrefine.org).

Default

Identification

Geographic Coverage

Bounding Box

Temporal Coverage

Range of Dates

Taxonomic Coverage

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

Taxonomic Classification

License Information

Distribution

Online

Additional Metadata

Metadata

GBIF Metadata Block

Project

• Project

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Personnel

Individual Name

Study Area Description

Design Description

Methods

• Method

Method Step

Method Step

Method Step

Method Step

Method Step

Method Step

Sampling

qualityControl