LifeWatch ERIC Metadata Catalogue
The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera
The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Default
Identification
- Alternate Identifier
- 10.15468/3vkdr2
- Alternate Identifier
- 50942caf-a62e-44c7-9998-e2a949aa85b2
- Alternate Identifier
- http://ipt.gbif.pt/ipt/resource?r=plecoptera_01
- Publication Date
- 2021-02-09
- Title
- The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera
- Abstract
- The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
- purpose
- The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
- Dataset Language
- ENGLISH
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Metadata Provider
- Dataset Contact
- Dataset Contact
- Keywords (n/a )
-
- Plecoptera
- Keywords (n/a )
-
- Occurrence records
- Keywords (n/a )
-
- Species distributions
- Keywords (n/a )
-
- Continental Portugal
- Keywords (n/a )
-
- Continental Spain
- Keywords (n/a )
-
- DNA barcode
- Keywords (n/a )
-
- Cytochrome c oxidase I (COI)
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
-
- Occurrence
- Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
-
- Specimen
- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
-
- Occurrence
Geographic Coverage
- Geographic Description
- Iberian Peninsula
Bounding Box
- West Bounding Coordinate
- -7.749
- East Bounding Coordinate
- 3.036
- North Bounding Coordinate
- 42.506
- South Bounding Coordinate
- 36.617
Temporal Coverage
Range of Dates
- Begin Date
-
2004-06-22
- End Date
-
2018-05-19
Taxonomic Coverage
- General Taxonomic Coverage
- This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.
Taxonomic Classification
- Taxonomic Rank Name
- Kingdom
- Taxonomic Rank Value
- Animalia
Taxonomic Classification
- Taxonomic Rank Name
- Phylum
- Taxonomic Rank Value
- Arthropoda
Taxonomic Classification
- Taxonomic Rank Name
- Class
- Taxonomic Rank Value
- Insecta
Taxonomic Classification
- Taxonomic Rank Name
- Order
- Taxonomic Rank Value
- Plecoptera
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Capniidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Chloroperlidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Leuctridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Nemouridae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Perlidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Perlodidae
Taxonomic Classification
- Taxonomic Rank Name
- Family
- Taxonomic Rank Value
- Taeniopterygidae
License Information
- Intellectual Rights
-
This work is licensed under a
Creative Commons Attribution (CC-BY) 4.0 License
.
Distributiondocument
Projects
Projects
• ProjectInBIO Barcoding Initiative
- Title
- The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera
Personnel
Individual Name
- Given Name
- Sónia
- Surname
- Ferreira
- User ID
- 0000-0002-6884-3966
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- J. Manuel
- Surname
- Tierno de Figueroa
- User ID
- 0000-0003-1616-9815
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Filipa
- Surname
- Martins
- User ID
- 0000-0003-4191-5031
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Joana
- Surname
- Veríssimo
- User ID
- 0000-0002-8721-9376
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Lorenzo
- Surname
- Quaglietta
- User ID
- 0000-0002-9703-0171
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- José M.
- Surname
- Grosso-Silva
- User ID
- 0000-0002-1094-0771
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Pedro Brito
- Surname
- Lopes
- User ID
- 0000-0002-5078-0602
- Role
- AUTHOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Sousa
- User ID
- 0000-0002-5859-9656
- Role
- ORIGINATOR
Personnel
Individual Name
- Given Name
- Pedro
- Surname
- Beja
- User ID
- 0000-0001-8164-0760
- Role
- AUTHOR
- Abstract
- The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
- Funding
- This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017.
Study Area Description
- Descriptor
-
Iberian Peninsula.
Design Description
- Description
- Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded.
Methods
Methods
• Method
Method Step
- Description
- Specimens were collected in 40 different localities of the Iberian Peninsula. Fieldwork was carried out from 2004 to 2018.
Method Step
- Description
- Specimens were collected during fieldwork by direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Collected specimens were stored in 96% ethanol.
Method Step
- Description
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature.
Method Step
- Description
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
Method Step
- Description
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
Method Step
- Description
- Data was submitted to GBIF, after being checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Sampling
- Study Extent
-
Iberian Peninsula.
- Sampling Description
- The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).
qualityControl
- Description
- All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org).
