Metadata Catalogue Metadata Catalogue https://metadatacatalogue.lifewatch.eu/ https://metadatacatalogue.lifewatch.eu//srv/eng/portal.opensearch https://metadatacatalogue.lifewatch.eu//srv/eng/rdf.search?any= eng Continents, countries, sea regions of the world. https://metadatacatalogue.lifewatch.eu//srv/eng/thesaurus.download?ref=external.place.regions ecoportal.fos_1.rdf https://metadatacatalogue.lifewatch.eu//srv/eng/thesaurus.download?ref=external.ecoportal.fos application/xml XML text/html HTML 57a42fde-aa63-4982-a7b7-05daed8a7e55 Rhea: R scripts for the analysis of microbial profiles Rhea: R scripts for the analysis of microbial profiles The importance of 16S rRNA gene amplicon profiles for understanding the influence of microbes in a variety of environments coupled with the steep reduction in sequencing costs led to a surge of microbial sequencing projects. Among available pipeline options for high-throughput 16S rRNA gene analysis, the R programming language and software environment for statistical computing stands out for its power and increased flexibility, and the possibility to adhere to most recent best practices and to adjust to individual project needs. The Rhea pipeline is a set of R scripts that encode a series of well-documented choices for the downstream analysis of Operational Taxonomic Units (OTUs) tables, including normalisation steps, alpha- and beta-diversity analysis, taxonomic composition, statistical comparisons, and calculation of correlations. Rhea is primarily a straightforward starting point for beginners, but can also be used as a framework for advanced users who can modify and expand the tool. Rhea is composed of 6 steps that can be run independently or as a set. Normalization Alpha-Diversity Beta-Diversity Taxonomic-Binning Serial-Group-Comparisons Correlations data management metagenomics taxonomy rRNA ASSEMBLE Plus R package calculation tool bioinformatics WWW:LINK-1.0-http--link https://lagkouvardos.github.io/Rhea/ WWW:LINK-1.0-http--link https://github.com/Lagkouvardos/Rhea license https://github.com/Lagkouvardos/Rhea/blob/master/LICENSE IMBCC-HCMR Ilias Lagkouvardos