Seasonal pigment concentration data of six European coastal wetlands under different ecological status measured spectrophotometrically
and using HPLC
This dataset includes pigment concertation data analysed during four seasonal samplings covering Autum 2023 through Summer 2024 in 6 different casepilot wetlands and 3 conservation status (altered, preserved, restored), that is two sites per wetland-conservation status combination. Pigment concentrations were determined spectrophotometrically and using HPLC technique. Data will be available after 31/12/2026.
Default
IdentificationAbout this resource
- Alternate Identifier
-
6160762c-2305-41f1-83bb-8c71bc385cd5
- Publication Date
- 2027-01-01
- Title
-
Seasonal pigment concentration data of six European coastal wetlands under different ecological status measured spectrophotometrically and using HPLC
- Abstract
-
This dataset includes pigment concertation data analysed during four seasonal samplings covering Autum 2023 through Summer 2024 in 6 different casepilot wetlands and 3 conservation status (altered, preserved, restored), that is two sites per wetland-conservation status combination. Pigment concentrations were determined spectrophotometrically and using HPLC technique. Data will be available after 31/12/2026.
- Dataset Language
-
English
- Dataset Creator
- Dataset Creator
- Dataset Creator
- Metadata Provider
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- Dataset Contact
- Dataset Contact
- Keywords (INRAE Thesaurus)
-
-
pigment analysis
-
spectrophotometry
-
high pressure liquid chromatography
-
- Keywords (Biodiversity Thesaurus)
-
-
wetland
-
Geographic Coverage
- Geographic Description
-
Danube Delta
Bounding Box
- West Bounding Coordinate
-
28.2843
- East Bounding Coordinate
-
29.7459
- North Bounding Coordinate
-
45.4723
- South Bounding Coordinate
-
44.8032
Geographic Coverage
- Geographic Description
-
Ria de Aveiro
Bounding Box
- West Bounding Coordinate
-
-8.72507
- East Bounding Coordinate
-
-8.59663
- North Bounding Coordinate
-
40.74963
- South Bounding Coordinate
-
40.63271
Geographic Coverage
- Geographic Description
-
Marjal dels moros
Bounding Box
- West Bounding Coordinate
-
-0.27997
- East Bounding Coordinate
-
-0.240362
- North Bounding Coordinate
-
39.64107
- South Bounding Coordinate
-
39.61579
Geographic Coverage
- Geographic Description
-
Curonian lagoon
Bounding Box
- West Bounding Coordinate
-
20.5142
- East Bounding Coordinate
-
21.2653
- North Bounding Coordinate
-
55.7186
- South Bounding Coordinate
-
54.8743
Geographic Coverage
- Geographic Description
-
Camargue
Bounding Box
- West Bounding Coordinate
-
4.4382
- East Bounding Coordinate
-
4.9275
- North Bounding Coordinate
-
43.674
- South Bounding Coordinate
-
43.3229
Geographic Coverage
- Geographic Description
-
Dutch wetlands
Bounding Box
- West Bounding Coordinate
-
3.9907
- East Bounding Coordinate
-
4.4087
- North Bounding Coordinate
-
51.6811
- South Bounding Coordinate
-
51.2754
Temporal Coverage
Range of Dates
- Begin Date
-
2023-10-01
- End Date
-
2024-08-31
resourceLicensesLicense Information
- Intellectual Rights
Distribution
Online
- A brief description of the the content of online URL.
-
DOI
Project
• Project
- Title
-
RESTORE4Cs - Modelling RESTORation of wEtlands for Carbon pathways, Climate Change mitigation and adaptation, ecosystem services, and biodiversity, Co-benefits
Personnel
Individual Name
- Given Name
-
Diana
- Surname
-
Vaičiūtė
- Organization Name
-
Marine Research Institute, Klaipeda University
- Position Name
-
Lead researcher
- User ID
-
0000-0003-4479-5688
- Role
-
Principal Investigator
Personnel
Individual Name
- Given Name
-
Jolita
- Surname
-
Petkuvienė
- Organization Name
-
Marine Research Institute, Klaipeda University
- Position Name
-
Senior researcher
- User ID
-
0000-0001-9015-9899
- Role
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Point Of Contact
- Abstract
-
All major European Union (EU) policies recognise the key role of wetlands to achieve the EU objectives regarding climate neutrality, biodiversity protection, zero-pollution, flood protection, and circular economy. Therefore, assessing the current extent and state of European wetlands, their current and potential greenhouse gases (GHG) profile and their medium to long-term mitigation capacity through restoration, or other measures, are key priorities of the European Union to tackle climate change. Led by the University of Aveiro, the Horizon project RESTORE4Cs (Modelling RESTORation of wEtlands for Carbon pathways, Climate Change mitigation and adaptation, ecosystem services, and biodiversity, Co-benefits) will assess the role of restoration action on wetlands capacity in terms of climate change mitigation and a wide range of ecosystem services using an integrative socio-ecological systems approach.
- Funding
-
EU Horizon 2.5.1 - Climate science and solutions
Methods
• Method
Method Step
- Description
-
Glass fibre filters extracted in acetone (100%). For extraction samples are placed into the refrigerator for 24 h. Samples are disrupted with an ultrasonic bath. Extracts are clarified using 0.2-μm, PTFE, HPLC syringe cartridge filters fitted with glass fiber prefilters.
Method Step
- Description
-
Analysis with HPLC system. Part of the extract was taken for the spectrophotometric analysis. Absorption was measure at 750, 665, 664, 647 and 630 nm wavelenght. Chlorophyll-a (Chl-a, uncorrected for pheopigments) was estimated using the trichromatic method of Jeffrey and Humphrey (1975). To obtain Chl-a corrected for pheopigments, as well as pheopigment concentrations, the extracts were measured before and after acidification with 1 N HCl, following the procedure of Lorenzen (1967).
Data Tables
• Data Table
- Name
-
RESTORE4Cs_PigmentsData.csv
Physical
- Object Name
-
RESTORE4Cs_PigmentsData.csv
- Size
-
59868
- Authentication
-
MD5:5799c39a16e0efe3de80c85ffae357bb
Data Format
- textFormat
-
1
column
,
Attribute List
Attribute
- Name
-
eventID
- Label
-
Event ID
- Definition
-
An identifier for the set of information associated with a dwc:Event (something that occurs at a place and time). May be a global unique identifier or an identifier specific to the data set.
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
Attribute
- Name
-
higherGeography
- Label
-
Higher Geography
- Definition
-
Full name of wetland casepilot where the sample was taken.
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
Attribute
- Name
-
locationID
- Label
-
Location ID
- Definition
-
Code that identifies the location (the wetland "unit") where the measurement was taken. Coded as a combination of the casepilot (higherGeography) abreviation and one of "A1", "A2", "P1", "P2", "R1" or "R2". Letters A, P, R followed by number correspond to the ecological status classification of the locations (A=Altered, P=Preserved, R= Restored).
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
Attribute
- Name
-
eventDate
- Label
-
Event Date
- Definition
-
The date-time or interval during which a dwc:Event occurred.
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
Attribute
- Name
-
ChlaWithoutCorrectionPhaeopigments
- Label
-
Chl-a without correction for phaeopigments
- Definition
-
Sum of extracted chlorophyll measurements on each filter of a filter cascade down to a GF/F filter. Each measurement was a spectrophotometric assay of an acetone extract following the Jeffrey and Humphrey protocols.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
Attribute
- Name
-
ChlaWithCorrectionPhaeopigments
- Label
-
Chl-a with correction for phaeopigments
- Definition
-
Sum of extracted chlorophyll measurements on each filter of a filter cascade down to a GF/F filter. Each measurement was a spectrophotometric assay of an acetone extract following the Jeffrey and Humphrey protocols.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
Attribute
- Name
-
19HEX
- Label
-
19'-hex fucoxanth
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
ACAR
- Label
-
a-carotene
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
ALLO
- Label
-
Alloxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
BCAR
- Label
-
b-carotene
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
CHLA
- Label
-
Chlorophyll-a
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
CHLB
- Label
-
Chlorophyll-b
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
CHLC2
- Label
-
Chlorophyll-c2
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
CHLC3
- Label
-
Chlorophyll-c3
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
DIAD
- Label
-
Diadinoxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
DIAT
- Label
-
Diatoxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
DVCHLA
- Label
-
Divinyl Chlorophyll-a
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
ECHI
- Label
-
Echinenone
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
FUCO
- Label
-
Fucoxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
LUTE
- Label
-
Lutein
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
NEOX
- Label
-
Neoxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
nominal
nonNumericDomain
textDomain
- Definition
-
Any Text
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
PERI
- Label
-
Peridinin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
PRAS
- Label
-
Prasinoxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
VIOL
- Label
-
Violaxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
ZEAX
- Label
-
Zeaxanthin
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND; DL
Pigments not detected; Result below the limit of detection of the analytical method
Attribute
- Name
-
CHLIA
- Label
-
Chlorophyllide-a
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
PHBA
- Label
-
Pheophorbide-a
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
Attribute
- Name
-
PHAE
- Label
-
Pheophytin-a
- Definition
-
The amount (mass or moles) of the specified pigment determined by HPLC assay of a sample collected by dissolution in acetone of the residue collected by GF/F filtration of a known volume of any water body. The quoted value either results from a single determination or the average of replicate determinations.
Measurement Scale
Ratio
Unit
- standardUnit
-
microgramsPerLiter
Numeric domain
- Number type
-
real
- Missing Value Code
-
ND
Pigments not detected
- numberOfRecords
-
439
