The InBIO Barcoding Initiative Database: Blattodea 01

The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Default

Identification

Alternate Identifier

64704ea2-6caf-40fd-a323-641b536f5152

Publication Date
2020-12-14
Title

The InBIO Barcoding Initiative Database: Blattodea 01

Abstract

The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Dataset Language

ENGLISH

 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Creator
  Zoologische Staatssammlung München - Horst Bohn (Professor)

Staatliche Naturwissenschaftliche Sammlungen Bayerns (SNSB), Zoologische Staatssammlung München, Münchhausenstraße 21

,

Munich

,

Bavaria

,

81247 München

,

GERMANY

Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
  Zoologische Staatssammlung München - Horst Bohn (Professor)

Staatliche Naturwissenschaftliche Sammlungen Bayerns (SNSB), Zoologische Staatssammlung München, Münchhausenstraße 21

,

Munich

,

Bavaria

,

81247 München

,

GERMANY

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Keywords (n/a)

  • Blattodea

Keywords (n/a)

  • Cockroach

Keywords (n/a)

  • Termite

Keywords (n/a)

  • Species

Keywords (n/a)

  • Cytochrome c oxidase I (COI)

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml)

  • Occurrence

Keywords (n/a)

  • DNA barcode

Keywords (n/a)

  • Continental Portugal

Geographic Coverage

Geographic Description

Continental Portugal.

Bounding Box

West Bounding Coordinate

-8.917

East Bounding Coordinate

-6.561

North Bounding Coordinate

41.892

South Bounding Coordinate

37.176

Temporal Coverage

Range of Dates

Begin Date

2005-08-15

End Date

2019-05-28

Taxonomic Coverage

General Taxonomic Coverage

This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.

Taxonomic Classification

Taxonomic Rank Name

Kingdom

Taxonomic Rank Value

Animalia

Taxonomic Classification

Taxonomic Rank Name

Phylum

Taxonomic Rank Value

Arthropoda

Taxonomic Classification

Taxonomic Rank Name

Class

Taxonomic Rank Value

Insecta

Taxonomic Classification

Taxonomic Rank Name

Order

Taxonomic Rank Value

Blattodea

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Blattidae

Taxonomic Classification

Taxonomic Rank Value

Ectobiidae

Taxonomic Classification

Taxonomic Rank Value

Rhinotermitidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 

Distribution

Online

URL
http://www.boldsystems.org
 

Additional Metadata

Metadata

GBIF Metadata Block

Date Stamp

2021-11-29T09:02:17Z

Citation

Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/m7f8sg accessed via GBIF.org on 2021-11-29.

Logo URL
https://cibio.up.pt/upload/image/logotipos/2-logo.png
Collection

CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970

InBIO Barcoding Initiative

Speciment Preservation Method

ALCOHOL

JGTI Curatorial Unit

Voucher tube

1

38

JGTI Curatorial Unit

DNA extractions

1

28

 

Project

• Project

Title

The InBIO Barcoding Initiative Database: Blattodea 01

Personnel

Individual Name

Given Name

Pedro

Surname

Beja

User ID

0000-0001-8164-0760

Role

AUTHOR

Personnel

Individual Name

Given Name

Sónia

Surname

Ferreira

User ID

0000-0002-6884-3966

Role

AUTHOR

Personnel

Individual Name

Given Name

Horst

Surname

Bohn

Role

AUTHOR

Personnel

Individual Name

Given Name

Pedro

Surname

Sousa

User ID

0000-0002-5859-9656

Role

ORIGINATOR

Abstract

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Funding

InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).

Study Area Description

Descriptor

Continental Portugal

Design Description

Description

Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%).

 
 

Methods

• Method

Method Step

Description

Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.

Method Step

Description

Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).

Method Step

Description

Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.

Method Step

Description

DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.

Method Step

Description

All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.

Method Step

Description

Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org/).

Sampling

Study Extent

Continental Portugal.

Sampling Description

The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.

qualityControl

Description

All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org).