Metadata Catalogue Metadata Catalogue https://metadatacatalogue.lifewatch.eu/ https://metadatacatalogue.lifewatch.eu//srv/eng/portal.opensearch https://metadatacatalogue.lifewatch.eu//srv/eng/rdf.search?any= eng Continents, countries, sea regions of the world. https://metadatacatalogue.lifewatch.eu//srv/eng/thesaurus.download?ref=external.place.regions ecoportal.fos_1.rdf https://metadatacatalogue.lifewatch.eu//srv/eng/thesaurus.download?ref=external.ecoportal.fos application/xml XML text/html HTML 7fadba9a-dcc3-40c8-b359-c07f094be68c DNA-based trends for the arrival of new non-indigenous taxa This workflow aims to detect non-indigenous species entering pelagic habitats early on by using environmental DNA metabarcoding. The biological samples used to develop the index are those collected using Autonomous Reef Monitoring Structures (ARMS; Kuwahara et al. 2013) by the EMOBON network. Samples are processed through the PEMA pipeline (Zafeiropoulos et al 2020), which produces a metabarcoding matrix of operational taxonomic units (OTUs). The resulting sequences are lists for each sampling station and these lists are then cross-checked against the WoRMS (Costello et al. 2013) catalogue of historical and regional occurrence records to pinpoint taxa absent from prior observations in the area from the WRiMS invasive checker web service (https:/www.marinespecies.org/introduced/news.php?p=showamp;id=8942). Newly detected non-indigenous species are flagged, yielding a time series that tracks the rate at which novel taxa appear. DNA-base metabarcoding pelagic habitats ARMS EMOBON PEMA WoRMS taxonomy WRiMS non-indigenous species WWW:LINK-1.0-http--link https://my.lifewatch.eu/workflow/create-workflow-from-template/aneris-arrival license LifeWatch ERIC Jose Zaiño