7fadba9a-dcc3-40c8-b359-c07f094be68c 1.0 DNA-based trends for the arrival of new non-indigenous taxa 2025-08-01 This workflow aims to detect non-indigenous species entering pelagic habitats early on by using environmental DNA metabarcoding. The biological samples used to develop the index are those collected using Autonomous Reef Monitoring Structures (ARMS; Kuwahara et al. 2013) by the EMOBON network. Samples are processed through the PEMA pipeline (Zafeiropoulos et al 2020), which produces a metabarcoding matrix of operational taxonomic units (OTUs). The resulting sequences are lists for each sampling station and these lists are then cross-checked against the WoRMS (Costello et al. 2013) catalogue of historical and regional occurrence records to pinpoint taxa absent from prior observations in the area from the WRiMS invasive checker web service (https:/www.marinespecies.org/introduced/news.php?p=show&id=8942). Newly detected non-indigenous species are flagged, yielding a time series that tracks the rate at which novel taxa appear. Jose Zaiño LifeWatch ERIC josea.zaino@lifewatch.eu 1.0.0 https://metadatacatalogue.lifewatch.eu/srv/api/records/7fadba9a-dcc3-40c8-b359-c07f094be68c/attachments/DNA-based%20trends%20for%20the%20arrival%20of%20new%20non-indigenous%20taxa.png DNA-based trends for the arrival of new non-indigenous taxa.png DNA-base metabarcoding pelagic habitats ARMS EMOBON PEMA WoRMS taxonomy WRiMS non-indigenous species GPLv3 - https://www.gnu.org/licenses/gpl-3.0.html https://my.lifewatch.eu/workflow/create-workflow-from-template/aneris-arrival WWW:LINK-1.0-http--link Link to the workflow PEMA Runner This service aims at running PEMA on all the sequences passed as input. It also requires a parameter file (a tsv file). https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/dec7b713-9757-4941-8632-a9ed73a2c4a1 PEMA Converter This service aims to reformat and produce final output in various formats for human and machine2machine reading. https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/b2424f84-1eba-4cb6-9798-f4275fd24a97 PEMA Sequences Retriever This service aims to download the sequences files (fasq files) in order to run PEMA. https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/4d149fe6-5317-41c8-adde-f72e59d5511b ARMS OTU Unifier This service aims at creating an OTU table with species information that can be processed by WoRMS and WRIMS. https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/a4ff4000-dcb2-41a7-9c61-7bff4ae20507 ARMS WoRMS Taxonomic Checker WoRMS taxon match webservice to check for the WoRMS match. https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/e9cb479f-9a36-4504-8d59-dd28520cec70 ARMS WRiMS Invasive Checker WRIMS taxon match webservice to check the known distribution of the species. https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/8df70810-490d-4dfd-964f-b73f28e34ba5 Metamds Observations https://metadatacatalogue.lifewatch.eu/srv/eng/catalog.search#/metadata/7d1c859a-970d-427f-8b12-f4c7f3b1fb25 https://helpdesk.lifewatch.eu