928e62a7-438f-4a66-a9ee-97a4f82f7007 Sarah Boulamail National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Research fellow sarahboulamail@cnr.it https://orcid.org/0000-0001-7105-6704 Lara Marastella Fumarola University of Salento PhD student laramarastella.fumarola@unisalento.it https://orcid.org/0000-0001-8148-0365 Francesco Cozzoli National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Researcher francesco.cozzoli@cnr.it https://orcid.org/0000-0002-6413-0977 Sarah Boulamail National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Research fellow sarahboulamail@cnr.it https://orcid.org/0000-0001-7105-6704 Cristina Di Muri National Research Council (CNR), Institute of Research on Terrestrial Ecosystems (IRET) Technologist cristina.dimuri@cnr.it https://orcid.org/0000-0003-4072-0662 Information Manager Martina Pulieri University of Salento Research assistant martina.pulieri@unisalento.it https://orcid.org/0009-0001-9691-1989 Information Manager 2025-04-07 English This dataset describes the metabolic responses of the groundwater crustacean Spelaeomysis bottazzii to abiotic factors in experimental conditions. The dataset includes variations of oxygen consumption and Standard Metabolic Rate (SMR) of 54 individuals of S. bottazzii under different temperatures (17°C, 21°C, and 25°C) and salinity levels (2, 4, and 6). Constant Volume Respirometry (CVR) was used to measure oxygen consumption across temperatures and salinity levels. Specimens' biomass and sex information are included in the dataset as such variables can modulate individuals' SMR. In addition, the growth rate of five different individuals of S. bottazzii was also evaluated at different time intervals over eight months. stygofauna AGROVOC abiotic factor climate change Biodiversity Thesaurus Experiment The Ecosystem Ontology metabolism metabolic rate growth rate Environmental Thesaurus Free for use, including sharing and adaptation, by all individuals provided that the owners are acknowledged in any use or publication. Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ Info and download page on LifeWatch Italy Data Portal https://data.lifewatchitaly.eu/handle/123456789/129683 Specimens of Spelaeomysis bottazzii used in this study have been collected from a cave in the locality of Sant’Isidoro (Nardò Municipality - Apulia, Italy). 17.929000 17.929000 40.222000 40.222000 2024-08-21 2024-12-14 Species Spelaeomysis bottazzii 226308 http://www.w3.org/ns/dcat#keyword http://vocabs.lter-europe.net/EnvThes/21317 http://www.w3.org/ns/dcat#keyword http://vocabs.lter-europe.net/EnvThes/21316 http://www.w3.org/ns/dcat#keyword http://data.loterre.fr/ark:/67375/BLH-WNMPKQ7Z-J http://www.w3.org/ns/dcat#keyword http://purl.dataone.org/odo/ECSO_00000505 http://www.w3.org/ns/dcat#keyword http://aims.fao.org/aos/agrovoc/c_87e413f9 http://www.w3.org/ns/dcat#keyword http://data.loterre.fr/ark:/67375/BLH-G57D2QQR-3 http://www.w3.org/ns/dcat#keyword http://vocabs.lter-europe.net/EnvThes/10060 Sarah Boulamail National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Research fellow sarahboulamail@cnr.it https://orcid.org/0000-0001-7105-6704 Francesco Cozzoli National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Researcher francesco.cozzoli@cnr.it https://orcid.org/0000-0002-6413-0977 LifeWatch Italy lifewatchitaly@gmail.com Plastic trays measuring 23 x 35 x 10 cm were used to house the animals throughout the experiment (ca. 18 individuals for tray), except for the specimens designated for growth rate measurements. The trays were inspected weekly to ensure both food and water were available. During the inspection, excreta and any remaining organic residues were removed using a pipette to keep the trays clean. The water level was maintained at a fixed mark, with the trays filled using a mixture of deionized and field water to contrast evaporation and keep the salinity consistently around 2. For feeding, the animals received a weekly ration consisting of 37 mg per capita of dry fish food containing 36% spirulina (Sera© Nature Micron) diluted into field water. Additionally, stones collected from the field were provided as environmental enrichment, offering natural hiding spots or resting areas. To evaluate standard aerobic metabolic rates under varying temperature and salinity conditions, Constant Volume Respirometry (CVR) assays were conducted on three groups of S. bottazzii, each consisting of 18 individuals with a similar size distribution (total: 54 specimens randomly selected from the field sample). After collection and acclimation to laboratory conditions, each group was maintained at a constant temperature of 17°C, 21°C, or 25°C for 2 weeks. After the first CVR assay at the natural salinity level (2), specimens were exposed in sequence to salinity levels of 4 and 6, each for 24 hours, and their oxygen consumption was measured at each salinity step. Thus, three repeated measurements at different salinity levels were obtained for each specimen, whereas no repeated measurements were taken across different temperatures. The specimens Respiration Rate (RR, mg h-1 ) was calculated from the slope (b) of the linear regression oxygen concentration in the respiration chamber (mg ml-1) over time (sec), so that RR = (b*V*3600). The obtained values were converted in Standard Metabolic Rate (SMR, J day-1), using an oxy-joule equivalent of 14.06 J mgO2-1. After measurements, each individual's Dry Weight (DW, mg) was determined by drying specimens at 60°C for 48 hours in an oven and weighing them using an analytical balance. CVR assays were conducted by using two 4-channel fiber-optic oxygen meters (FireSting O2, PyroScience GmbH, Regensburg, Germany) equipped with sensor spots attached to the inner walls of 0.8 mL (V) respiration chambers. To measure growth rates, six of the smallest sampled individuals were isolated in separate microcosms to allow individual recognition. These specimens were maintained in darkness, in natural water at 17°C and salinity of 2, mirroring the conditions at the collection site, and were fed ad libitum. Body length was measured regularly over 262 days. The specimens were placed in a transparent plastic glass 6 cm in diameter. The bottom of the containers were transparent and placed on a sheet of graph paper. Photographs of the individuals were taken at different time intervals: once per week initially, then every two weeks. All images were taken using an iPhone SE (2022), ensuring that the device was perpendicular to the container by using the built-in level to prevent image distortion. The growth rate was assessed by measuring the body length, defined as the distance from the anterior margin of the carapace to the posterior margin of the telson without spines, in each image using ImageJ (version 1.54g), with graph paper as reference scale. The specimens of Spelaeomysis bottazzii used in this study have been collected from a cave in the locality of Sant’Isidoro, (Nardò Municipality - Apulia, Italy) (40.222 N - 17.929 E). The site is located along the wide coastal plain of the Ionian Sea. The about 25 km-long coastal stretch ​​comprised between Torre Castiglione (Municipality of Porto Cesareo) to the north, and Serra Cicora (Municipality of Nardò) to the south, is characterized by numerous collapse dolines. Specimens of Spelaeomysis bottazzii were collected using a simple trapping method. Three weighted buckets were placed horizontally in the water with its opening facing the deeper part of the cave. Approximately 10 g of chicken liver bait was placed inside, attracting S. bottazzii to feed and remain in the bucket. By quickly lifting the buckets into a vertical position, the specimens were trapped. The trap was checked after 24 hours, and the captured animals were collected. S. bottazzii was the only species observed and collected at this site using this method. After field collection, the specimens were transported to the laboratory in an insulated thermos container and placed in plastic trays with field water to minimize potential osmoregulatory stress. Francesco Cozzoli National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Researcher francesco.cozzoli@cnr.it 0000-0002-6413-0977 Principal Investigator Sarah Boulamail National Research Council of Italy (CNR), Research Institute on Terrestrial Ecosystems (IRET) Research fellow sarahboulamail@cnr.it 0000-0001-7105-6704 Content Provider SpelaeomyisisBottazzii_StandardMetabolicRate_20250407.csv This dataset describes oxygen consumption (Respiration Rate) and Standard Metabolic Rate (SMR) of Spelaeomysis bottazzii individuals at different temperatures and salinities. SpelaeomyisisBottazzii_StandardMetabolicRate_20250407.csv 30715 MD5:29f985827bf14a03f013db87afe42aa1 1 column , catalogNumber Catalog Number An identifier (preferably unique) for the record within the data set or collection. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/catalogNumber organismID Organism ID An identifier for the dwc:Organism instance (as opposed to a particular digital record of the dwc:Organism). May be a globally unique identifier or an identifier specific to the data set. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/organismID acceptedNameUsage Accepted Name Usage The full name, with authorship and date information if known, of the currently valid (zoological) or accepted (botanical) dwc:Taxon. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/acceptedNameUsage scientificNameAuthorship Scientific Name Authorship The authorship information for the dwc:scientificName formatted according to the conventions of the applicable dwc:nomenclaturalCode. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/scientificNameAuthorship acceptedNameUsageID Accepted Name Usage ID An identifier for the name usage (documented meaning of the name according to a source) of the currently valid (zoological) or accepted (botanical) taxon. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/acceptedNameUsageID taxonRank Taxon Rank The taxonomic rank of the most specific name in the dwc:scientificName. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/taxonRank decimalLatitude Decimal Latitude The geographic latitude of the sampling site (in decimal degrees, using the spatial reference system given in dwc:geodeticDatum) of the geographic center of a dcterms:Location. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. degree ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/decimalLatitude decimalLongitude Decimal Longitude The geographic longitude of the sampling site (in decimal degrees, using the spatial reference system given in dwc:geodeticDatum) of the geographic center of a dcterms:Location. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. degree ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/decimalLongitude sex Sex The sex of the biological individual(s) represented in the dwc:Occurrence. nominal M A biological sex quality inhering in an individual or a population whose sex organs contain only male gametes F A biological sex quality inhering in an individual or a population that only produces gametes that can be fertilised by male gametes http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#ofCharacteristic http://rs.tdwg.org/dwc/terms/sex eventDate Event Date The date-time or interval during which a dwc:Event occurred. For occurrences, this is the date-time when the dwc:Event was recorded. Not suitable for a time in a geological context. dateTime YYYY-MM-DD http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/eventDate salinity salinity Salinity is the saltiness or dissolved salt content of a body of water (see also soil salinity). Salinity is an important factor in determining many aspects of the chemistry of natural waters and of biological processes within it, and is a thermodynamic state variable that, along with temperature and pressure, governs physical characteristics like the density and heat capacity of the water. PSU ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType http://vocabs.lter-europe.net/EnvThes/10312 dryWeight Dry weight The weight of an organism after removal of body fluids drying at 50-70 °C (typically at 60 °C) in a drying oven for 24 hours or until constant weight is reached. milligram ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType https://kos.lifewatch.eu/thesauri/traits/c_4d66d52a respirationRate Respiration rate The rate between oxygen consume and carbon dioxide release through cellular respiration. mgO2PerHour ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType https://kos.lifewatch.eu/thesauri/traits/c_3b6ce5dd waterTemperature water temperature Any measurable characteristic related to the temperature of water celsius ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType http://vocabs.lter-europe.net/EnvThes/22313 standardMetabolicRate Standard Metabolic Rate A measure of the rate of metabolic activity in a living organism. It is the rate at which an organism uses energy to sustain essential life processes such as respiration, growth, reproduction and, in animals, processes such as blood circulation, muscle tone and activity. It can be determined in numerous ways: (i) as the total heat produced over a given period, (ii) as oxygen consumption (and sometimes also carbon dioxide production) over a given period, which although easier to measure, only gives the contribution of aerobic metabolism, (iii) as the energy content of the food eaten over a given period, and (iv) by the fate of isotopically labelled water. see average daily metabolic rate, basal metabolic rate, energy budget, field metabolic rate, respiratory quotient, resting metabolic rate, standard metabolic rate. JoulePerDay ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType http://vocabs.lter-europe.net/EnvThes/msa0652 158 SpelaeomyisisBottazzii_GrowthRate_20250407.csv This dataset describes the growth rates of Spelaeomyisis bottazzii individuals in experimental conditions. SpelaeomyisisBottazzii_GrowthRate_20250407.csv 13558 MD5:3ac94cbda476098822b1032ad56fe0f2 1 column , catalogNumber Catalog Number An identifier (preferably unique) for the record within the data set or collection. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/catalogNumber organismID Organism ID An identifier for the dwc:Organism instance (as opposed to a particular digital record of the dwc:Organism). May be a globally unique identifier or an identifier specific to the data set. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/organismID acceptedNameUsage Accepted Name Usage The full name, with authorship and date information if known, of the currently valid (zoological) or accepted (botanical) dwc:Taxon. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/acceptedNameUsage scientificNameAuthorship Scientific Name Authorship The authorship information for the dwc:scientificName formatted according to the conventions of the applicable dwc:nomenclaturalCode. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/scientificNameAuthorship acceptedNameUsageID Accepted Name Usage ID An identifier for the name usage (documented meaning of the name according to a source) of the currently valid (zoological) or accepted (botanical) taxon. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasCode http://rs.tdwg.org/dwc/terms/acceptedNameUsageID taxonRank Taxon Rank The taxonomic rank of the most specific name in the dwc:scientificName. nominal http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/taxonRank decimalLatitude Decimal Latitude The geographic latitude of the sampling site (in decimal degrees, using the spatial reference system given in dwc:geodeticDatum) of the geographic center of a dcterms:Location. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. degree ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/decimalLatitude decimalLongitude Decimal Longitude The geographic longitude of the sampling site (in decimal degrees, using the spatial reference system given in dwc:geodeticDatum) of the geographic center of a dcterms:Location. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. degree ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/decimalLongitude eventDate Event Date The date-time or interval during which a dwc:Event occurred. For occurrences, this is the date-time when the dwc:Event was recorded. Not suitable for a time in a geological context. dateTime YYYY-MM-DD http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#hasContext http://rs.tdwg.org/dwc/terms/eventDate bodyLength Body length The total distance from point to point along the longest axis of the body of an organism. millimeter ratio http://ecoinformatics.org/oboe/oboe.1.2/oboe-core.owl#containsMeasurementsOfType https://kos.lifewatch.eu/thesauri/traits/c_d266fb02 86