The InBIO Barcoding Initiative Database: Hemiptera 01

The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Default

Identification

Alternate Identifier

963a25a6-839c-4bff-9889-97fc8385f564

Publication Date
2021-07-30
Title

The InBIO Barcoding Initiative Database: Hemiptera 01

Abstract

The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

purpose

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Dataset Language

ENGLISH

 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Creator
Dataset Creator
  Rui Andrade (Researcher)

Rua Calouste Gulbenkian, 237

,

Porto

,

Porto

,

4050-145

,

PORTUGAL

Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
Metadata Provider
  Rui Andrade (Researcher)

Rua Calouste Gulbenkian, 237

,

Porto

,

Porto

,

4050-145

,

PORTUGAL

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)

Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7

,

Vairão

,

Porto

,

4485-661

,

PORTUGAL

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml)

  • Hemiptera

Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml)

  • Species

Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml)

  • Occurrence

Keywords (n/a)

  • Cytochrome c oxidase I (COI)

Keywords (n/a)

  • DNA barcode

Keywords (n/a)

  • Continental Portugal

Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml)

  • Occurrence

Geographic Coverage

Geographic Description

Continental Portugal.

Bounding Box

West Bounding Coordinate

-9.465

East Bounding Coordinate

-6.344

North Bounding Coordinate

41.979

South Bounding Coordinate

37.257

Temporal Coverage

Range of Dates

Begin Date

2015-03-16

End Date

2019-09-20

Taxonomic Coverage

General Taxonomic Coverage

This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.

Taxonomic Classification

Taxonomic Rank Name

Kingdom

Taxonomic Rank Value

Animalia

Taxonomic Classification

Taxonomic Rank Name

Phylum

Taxonomic Rank Value

Arthropoda

Taxonomic Classification

Taxonomic Rank Name

Class

Taxonomic Rank Value

Insecta

Taxonomic Classification

Taxonomic Rank Name

Order

Taxonomic Rank Value

Hemiptera

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Acanthosomatidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Alydidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Aphalaridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Aphrophoridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Aradidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Auchenorrhyncha

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Berytidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Cercopidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Cicadellidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Cicadidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Coreidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Corixidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Cydnidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Delphacidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Dictyopharidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Flatidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Gerridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Heteroptera

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Hydrometridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Lygaeidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Membracidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Miridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Nabidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Nepidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Notonectidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Pentatomidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Potamocoridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Pyrrhocoridae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Reduviidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Rhopalidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Rhyparochromidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Stenocephalidae

Taxonomic Classification

Taxonomic Rank Name

Family

Taxonomic Rank Value

Thaumastocoridae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 

Distribution

Online

URL
http://www.boldsystems.org/index.php/Public_SearchTerms?query=DS-IBIHP01
 

Additional Metadata

Metadata

GBIF Metadata Block

Date Stamp

2021-11-29T09:07:15Z

Citation

Sousa P, Grosso-Silva J M, Andrade R, Beja P, Ferreira S (2021). The InBIO Barcoding Initiative Database: Hemiptera 01. Version 1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/8hn25y accessed via GBIF.org on 2021-11-29.

Bibliography

Sousa, P., Grosso-Silva, J.M., Andrade, R., Chaves, C., Pinto, J., Paupério, J., Beja, P. Ferreira, S. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Hemiptera 01. Biodiversity Data Journal, 9: e65314. doi: 10.3897/BDJ.9.e65314

Logo URL
https://cibio.up.pt/upload/image/logotipos/2-logo.png
Collection

4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970

InBIO Barcoding Initiative

Speciment Preservation Method

ALCOHOL

JGTI Curatorial Unit

Voucher tube

1

133

JGTI Curatorial Unit

DNA extractions

1

135

 

Project

• Project

Title

The InBIO Barcoding Initiative Database: Hemiptera 01

Personnel

Individual Name

Given Name

Sónia

Surname

Ferreira

User ID

0000-0002-6884-3966

Role

AUTHOR

Personnel

Individual Name

Given Name

José M.

Surname

Grosso-Silva

User ID

0000-0002-1094-0771

Role

AUTHOR

Personnel

Individual Name

Given Name

Rui

Surname

Andrade

Role

AUTHOR

Personnel

Individual Name

Given Name

Pedro

Surname

Sousa

User ID

0000-0002-5859-9656

Role

AUTHOR

Personnel

Individual Name

Given Name

Pedro

Surname

Beja

User ID

0000-0001-8164-0760

Role

AUTHOR

Abstract

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Funding

This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC).

Study Area Description

Descriptor

Specimens were collected in 12 continental Portugal districts. Sixty different localities were, almost half of which (47%) from the Bragança district. Sampling was conducted from 2015 to 2019, with 39% of the specimens collected in the year 2018.

Design Description

Description

Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens resulted from fieldwork collection by direct search of specimens or by sweeping the vegetation.

 
 

Methods

• Method

Method Step

Description

Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.

Method Step

Description

Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).

Method Step

Description

Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.

Method Step

Description

DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.

Method Step

Description

All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.

Method Step

Description

Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.

Sampling Description

The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.

qualityControl

Description

All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org).