The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01

The dataset contains 234 records of Lacewings (Neuroptera) species collected from 2006 to 2019 in continental Portugal (Oliveira et al., 2021). Specimens were detected and captured by direct search of the environment and by using both UV and mercury-vapor lamps to attract the insects. Captured specimens were preserved in 96% ethanol. All captured specimens were identified to species level. Samples of each species were selected for DNA sequencing based on their geographic provenance. From each specimen, one leg (tissue sample) was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Default

Identification

Alternate Identifier
10.15468/6cyrxd
Alternate Identifier
b63b377f-b7c9-450d-bee8-34056169ff25
Alternate Identifier
http://ipt.gbif.pt/ipt/resource?r=ibi_neuroptera_01
Publication Date
2021-08-06
Title
The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01
Abstract
The dataset contains 234 records of Lacewings (Neuroptera) species collected from 2006 to 2019 in continental Portugal (Oliveira et al., 2021). Specimens were detected and captured by direct search of the environment and by using both UV and mercury-vapor lamps to attract the insects. Captured specimens were preserved in 96% ethanol. All captured specimens were identified to species level. Samples of each species were selected for DNA sequencing based on their geographic provenance. From each specimen, one leg (tissue sample) was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Dataset Language
ENGLISH
 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Daniel Oliveira (Master’s Student )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  Martin Corley (Researcher )
Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Vanessa Alves Mata (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Brito Lopes (Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Daniel Oliveira (Master’s Student )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  Martin Corley (Researcher )
Pucketty Farm Cottage , Faringdon , Oxfordshire , SN7 8JP , UNITED_KINGDOM
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Vanessa Alves Mata (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Brito Lopes (Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 ,
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Keywords ((n/a) )
  • Neuroptera
Keywords ((n/a) )
  • Lacewings
Keywords ((n/a) )
  • Species
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
  • Occurrence
Keywords ((n/a) )
  • Cytochrome c oxidase I (COI)
Keywords ((n/a) )
  • DNA barcode
Keywords ((n/a) )
  • Continental Portugal
Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
  • Specimen

Geographic Coverage

Geographic Description
Continental Portugal

Bounding Box

West Bounding Coordinate
-9.46
East Bounding Coordinate
-6.31
North Bounding Coordinate
41.98
South Bounding Coordinate
37.17

Temporal Coverage

Range of Dates

Begin Date

2006-06-30

End Date

2019-09-20

Taxonomic Coverage

General Taxonomic Coverage
This dataset is composed of data relating to 234 Neuroptera specimens belonging to 50 species, that represent half of the species known from continental Portugal. These species are representative of nine of the ten families of Neuroptera known from continental Portugal. The families with the highest number of recorded specimens and species diversity are the Chrysopidae (87 records, 21 species), Myrmeleontidae (68 records, 12 species) and Hemerobiidae (42 records, 8 species).

Taxonomic Classification

Taxonomic Rank Name
Kingdom
Taxonomic Rank Value
Animalia

Taxonomic Classification

Taxonomic Rank Name
Phylum
Taxonomic Rank Value
Arthropoda

Taxonomic Classification

Taxonomic Rank Name
Class
Taxonomic Rank Value
Insecta

Taxonomic Classification

Taxonomic Rank Name
Order
Taxonomic Rank Value
Neuroptera

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Ascalaphidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Berothidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Chrysopidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Coniopterygidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Dilaridae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Hemerobiidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Mantispidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Myrmeleontidae

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Nemopteridae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 
 
 

Projects

Projects

• ProjectInBIO Barcoding Initiative

Title
The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01

Personnel

Individual Name

Given Name
Pedro
Surname
Beja
User ID
0000-0001-8164-0760
Role
AUTHOR

Personnel

Individual Name

Given Name
Sónia
Surname
Ferreira
User ID
0000-0002-6884-3966
Role
AUTHOR

Personnel

Individual Name

Given Name
Daniel
Surname
Oliveira
User ID
0000-0002-8733-4104
Role
AUTHOR

Personnel

Individual Name

Given Name
Martin
Surname
Corley
User ID
0000-0003-4240-8007
Role
AUTHOR

Personnel

Individual Name

Given Name
Luís P.
Surname
da Silva
User ID
0000-0003-2358-1277
Role
AUTHOR

Personnel

Individual Name

Given Name
Vanessa Alves
Surname
Mata
User ID
0000-0003-3005-9030
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro Brito
Surname
Lopes
User ID
0000-0002-5078-0602
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro
Surname
Sousa
User ID
0000-0002-5859-9656
Role
ORIGINATOR
Abstract
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
Funding
InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).

Study Area Description

Descriptor

Specimens were collected in 12 continental Portugal administrative districts. Eighty-seven different localities were sampled. Sampling was conducted from 2006 to 2019, although most specimens (87.6%) were collected in the period between 2017 to 2019.

Design Description

Description
The specimens in this dataset were collected in fieldwork incursions carried out between 2006 and 2019, encompassing several sites throughout continental Portugal in an attempt to have a good representation of the country’s Neuroptera fauna. Specimens were morphologically identified and DNA barcoded.
 
 

Methods

Methods

• Method

Method Step

Description
Specimens were collected in 87 different localities of continental Portugal. Fieldwork was carried out between 2006 and 2019, with 87.6% of the records obtained between 2017 and 2019.

Method Step

Description
Specimens were collected by direct search of the sampling site while also using both UV LED and mercury-vapor lamps as lures distributed along the site. Specimens were preserved in 96% alcohol and deposited in the IBI reference collection.

Method Step

Description
Specimens were morphologically identified using a stereoscopic microscope, an Olympus SZX2-ILLT, and resorting to most recent literature on Iberian Neuroptera (Monserrat, 2014a, 2014b, 2014c, 2016a, 2016b; Monserrat and Acevedo, 2013; Monserrat et al., 2012a; 2012b, 2014)

Method Step

Description
All specimens were DNA barcoded. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All tissue samples and DNA extracts were deposited in the IBI collection.

Method Step

Description
All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.

Method Step

Description
Prior to GBIF submission, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in 87 different localities of continental Portugal. Sampling was conducted from 2006 to 2019, in several fieldwork expeditions. Most specimens (87.6%) were collected in the period between 2017 to 2019.

Sampling Description
The studied material was collected in 87 different Portuguese localities. Sampling was conducted between 2006 and 2019. Specimens were captured by direct search of the environment and by using both UV LED and mercury-vapor lamps to attract the insects. Specimens were preserved in 96% ethanol for downstream molecular analysis and stored at the InBIO Barcoding Initiative (IBI )reference collection.

qualityControl

Description

To each specimen, the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.

Prior to GBIF submission, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

 
 

Not available