Metadata Catalogue
The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Default
Identification
- Alternate Identifier
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cd11c12e-1f1c-4ec6-8b28-c57425956915
- Publication Date
- 2021-01-05
- Title
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The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
- Abstract
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The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
- purpose
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The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
- Dataset Language
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ENGLISH
- Dataset Creator
- Dataset Creator
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CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Dataset Creator
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Metadata Provider
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Coordinating Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Metadata Provider
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Dataset Contact
-
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher)
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
,Vairão
,Porto
,4485-661
,PORTUGAL
- Keywords (n/a)
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Lepidoptera
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- Keywords (n/a)
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Species
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- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml)
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Occurrence
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- Keywords (n/a)
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Cytochrome c oxidase I (COI)
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- Keywords (n/a)
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DNA barcode
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- Keywords (n/a)
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Continental Portugal
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- Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml)
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Occurrence
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Geographic Coverage
- Geographic Description
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Continental Portugal.
Bounding Box
- West Bounding Coordinate
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-8.169
- East Bounding Coordinate
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-7.004
- North Bounding Coordinate
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42.003
- South Bounding Coordinate
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41.422
Temporal Coverage
Range of Dates
- Begin Date
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2012-06-14
- End Date
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2016-05-31
Taxonomic Coverage
- General Taxonomic Coverage
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This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.
Taxonomic Classification
- Taxonomic Rank Name
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Kingdom
- Taxonomic Rank Value
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Animalia
Taxonomic Classification
- Taxonomic Rank Name
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Phylum
- Taxonomic Rank Value
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Arthropoda
Taxonomic Classification
- Taxonomic Rank Name
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Class
- Taxonomic Rank Value
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Insecta
Taxonomic Classification
- Taxonomic Rank Name
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Order
- Taxonomic Rank Value
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Lepidoptera
Taxonomic Classification
- Taxonomic Rank Name
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Family
- Taxonomic Rank Value
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Tortricidae
License Information
- Intellectual Rights
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This work is licensed under a
Creative Commons Attribution (CC-BY) 4.0 License
.
Distribution
Additional Metadata
Metadata
GBIF Metadata Block
- Date Stamp
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2021-11-29T09:07:09Z
- Citation
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Corley M, Ferreira S, Sousa P, Beja P (2021). The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Version 1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/9z8bpm accessed via GBIF.org on 2021-11-29.
- Bibliography
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Corley, M.F.V. Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600.
Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3.
- Collection
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CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
InBIO Barcoding Initiative
- Speciment Preservation Method
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ALCOHOL
- JGTI Curatorial Unit
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Voucher tube
1
5
- JGTI Curatorial Unit
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DNA extractions
1
5
Project
• Project
- Title
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The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
Personnel
Individual Name
- Given Name
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Pedro
- Surname
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Beja
- User ID
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0000-0001-8164-0760
- Role
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AUTHOR
Personnel
Individual Name
- Given Name
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Martin
- Surname
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Corley
- User ID
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0000-0003-4240-8007
- Role
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AUTHOR
Personnel
Individual Name
- Given Name
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Sónia
- Surname
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Ferreira
- User ID
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0000-0002-6884-3966
- Role
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AUTHOR
Personnel
Individual Name
- Given Name
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Pedro
- Surname
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Sousa
- User ID
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0000-0002-5859-9656
- Role
-
ORIGINATOR
- Abstract
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The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
- Funding
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InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).
Study Area Description
- Descriptor
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Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016.
Design Description
- Description
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Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night.
Methods
• Method
Method Step
- Description
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Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
Method Step
- Description
-
The captured specimens were preserved in dry storage.
Method Step
- Description
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The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
Method Step
- Description
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DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
Method Step
- Description
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The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
Method Step
- Description
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Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org/).
Sampling
- Study Extent
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Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016.
- Sampling Description
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Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
qualityControl
- Description
-
The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 ( http://openrefine.org/).