The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)

The dataset contains 11 records of Stag beetle (Lucanidae) species collected from 2015 to 2019 in continental Portugal. All specimens were identified to species level, and belong to 3 species of the Lucanidae (Coleoptera). Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (a leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences available were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Default

Identification

Alternate Identifier
10.15468/7559ru
Alternate Identifier
f1f1225f-4247-43a3-83de-7fbd2dca7e92
Alternate Identifier
http://ipt.gbif.pt/ipt/resource?r=ibi_lucanidae
Publication Date
2021-11-11
Title
The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)
Abstract
The dataset contains 11 records of Stag beetle (Lucanidae) species collected from 2015 to 2019 in continental Portugal. All specimens were identified to species level, and belong to 3 species of the Lucanidae (Coleoptera). Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (a leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences available were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
purpose
The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Dataset Language
ENGLISH
 
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Creator
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Luís P. da Silva (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Sousa (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Metadata Provider
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Pedro Beja (Coordinating Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Dataset Contact
  CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto - Sónia Ferreira (Post-Doc Researcher )
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 , Vairão , Porto , 4485-661 , PORTUGAL
Keywords (n/a )
  • Coleoptera
Keywords (n/a )
  • Lucanidae
Keywords (n/a )
  • Species
Keywords (n/a )
  • Cytochrome c oxidase I (COI)
Keywords (n/a )
  • DNA barcode
Keywords (n/a )
  • Continental Portugal
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml )
  • Occurrence
Keywords (GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml )
  • Specimen
Keywords (GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml )
  • Occurrence

Geographic Coverage

Geographic Description
Continental Portugal

Bounding Box

West Bounding Coordinate
-9
East Bounding Coordinate
-6.7
North Bounding Coordinate
41.9
South Bounding Coordinate
37

Temporal Coverage

Range of Dates

Begin Date

2015-06-17

End Date

2019-09-19

Taxonomic Coverage

General Taxonomic Coverage
This dataset is composed of data relating to 11 Lucanidae (Coleoptera) specimens. All specimens were determined to species level. Three of the four Stag beetle species known to occur in continental Portugal are represented in the dataset, with Dorcus parallelipipedus as the most represented species in the dataset, accounting for seven of the eleven specimens.

Taxonomic Classification

Taxonomic Rank Name
Kingdom
Taxonomic Rank Value
Animalia

Taxonomic Classification

Taxonomic Rank Name
Phylum
Taxonomic Rank Value
Arthropoda

Taxonomic Classification

Taxonomic Rank Name
Class
Taxonomic Rank Value
Insecta

Taxonomic Classification

Taxonomic Rank Name
Order
Taxonomic Rank Value
Coleoptera

Taxonomic Classification

Taxonomic Rank Name
Family
Taxonomic Rank Value
Lucanidae

License Information

Intellectual Rights

This work is licensed under a

Creative Commons Attribution (CC-BY) 4.0 License

.

 

Distributiondocument

Online

URL
http://boldsystems.org
 
 

Projects

Projects

• ProjectInBIO Barcoding Initiative

Title
The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)

Personnel

Individual Name

Given Name
Pedro
Surname
Beja
User ID
0000-0001-8164-0760
Role
AUTHOR

Personnel

Individual Name

Given Name
Pedro
Surname
Sousa
User ID
0000-0002-5859-9656
Role
ORIGINATOR

Personnel

Individual Name

Given Name
Luís
Surname
P. da Silva
User ID
0000-0003-2358-1277
Role
AUTHOR

Personnel

Individual Name

Given Name
Sónia
Surname
Ferreira
User ID
0000-0002-6884-3966
Role
AUTHOR
Abstract
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
Funding
InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).

Study Area Description

Descriptor

Specimens were collected in five Portugal districts and a total of ten sites were sampled. Sampling was conducted from 2015 to 2019.

Design Description

Description
Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens were located by direct searches carried out during field work, which enabled the sampling of both live and dead specimens.
 
 

Methods

Methods

• Method

Method Step

Description
Sampling was conducted in continental Portugal from 2015 and 2019, and consisted in direct search. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed from which DNA was extracted and the COI DNA barcode fragment was sequenced. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Sampling

Study Extent

Specimens were collected in ten localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.

Sampling Description
Specimens were collected in ten localities of continental Portugal. Sampling was conducted between 2015 and 2019, by direct search. Collected specimens were identified using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis.

qualityControl

Description
To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.
 
 

Not available