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  <dataset>
    <alternateIdentifier>oai:marineinfo.org:id:dataset:20</alternateIdentifier>
    <title xml:lang="en">Chemical analysis of PAK's, Organotin, PCB's, PBDE's and organochlorine pesticides as possible endocrine disruptors in Scheldt estuary</title>
    <creator>
      <individualName>
        <givenName>Els</givenName>
        <surName>Monteyne</surName>
      </individualName>
      <organizationName>Koninklijk Belgisch Instituut voor Natuurwetenschappen; Departement Beheer van het Mariene Ecosysteem; Beheerseenheid Mathematisch Model Noordzee en Schelde-estuarium; Oostende</organizationName>
    </creator>
    <creator>
      <individualName>
        <givenName>Patrick</givenName>
        <surName>Roose</surName>
      </individualName>
      <organizationName>Koninklijk Belgisch Instituut voor Natuurwetenschappen; Departement Beheer van het Mariene Ecosysteem; Beheerseenheid Mathematisch Model Noordzee en Schelde-estuarium; Oostende</organizationName>
    </creator>
    <metadataProvider>
      <organizationName>Flanders Marine Institute (VLIZ)</organizationName>
      <address>
        <country>BE</country>
      </address>
      <electronicMailAddress>info@vliz.be</electronicMailAddress>
      <onlineUrl>https://www.vliz.be</onlineUrl>
    </metadataProvider>
    <associatedParty>
      <individualName>
        <givenName>Els</givenName>
        <surName>Monteyne</surName>
      </individualName>
      <organizationName>Koninklijk Belgisch Instituut voor Natuurwetenschappen; Departement Beheer van het Mariene Ecosysteem; Beheerseenheid Mathematisch Model Noordzee en Schelde-estuarium; Oostende</organizationName>
      <role>custodianSteward</role>
    </associatedParty>
    <pubDate>2021-12-02</pubDate>
    <language>en</language>
    <abstract>
      <para>Water, sediment, suspended matter and biota were sampled along the Western Scheldt from 2002 to 2006. All matrices were analysed for PAK's, Organotin, PCB's, PBDE's and OCl pesticides using GCMS and GCMS/MS.</para>
    </abstract>
    <keywordSet>
      <keyword>Chemical pollutants</keyword>
      <keyword>Chlorinated hydrocarbons</keyword>
      <keyword>DDT</keyword>
      <keyword>Estuaries</keyword>
      <keyword>Halogenated hydrocarbons</keyword>
      <keyword>PCB</keyword>
      <keyword>Pesticides</keyword>
      <keyword>Polychlorinated biphenyls</keyword>
      <keyword>Toxicants</keyword>
      <keyword>Tributyltin</keyword>
      <keyword>Water quality</keyword>
      <keywordThesaurus>ASFA</keywordThesaurus>
    </keywordSet>
    <intellectualRights>
      <para>
        This work is licensed under a
        <ulink url="http://creativecommons.org/licenses/by/4.0/legalcode">
          <citetitle>Creative Commons Attribution (CC-BY) 4.0 License</citetitle>
        </ulink>
        .
      </para>
    </intellectualRights>
    <coverage>
      <geographicCoverage id="http://marineregions.org/mrgid/4812">
        <geographicDescription>ANE, Scheldt Estuary</geographicDescription>
      </geographicCoverage>
      <geographicCoverage>
        <geographicDescription>EurOBIS calculated BBOX &gt; station Bounding Box</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>3.5699999332428</westBoundingCoordinate>
          <eastBoundingCoordinate>4.3916602134705</eastBoundingCoordinate>
          <northBoundingCoordinate>51.436668395996</northBoundingCoordinate>
          <southBoundingCoordinate>51.21883392334</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/14660">
        <geographicDescription>DAS20_S12 &gt; station S12</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>4.225</westBoundingCoordinate>
          <eastBoundingCoordinate>4.225</eastBoundingCoordinate>
          <northBoundingCoordinate>51.365</northBoundingCoordinate>
          <southBoundingCoordinate>51.365</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/9106">
        <geographicDescription>DAS20_S07 &gt; station S07</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>4</westBoundingCoordinate>
          <eastBoundingCoordinate>4</eastBoundingCoordinate>
          <northBoundingCoordinate>51.43666667</northBoundingCoordinate>
          <southBoundingCoordinate>51.43666667</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/213">
        <geographicDescription>DAS20_S04 &gt; station S04</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>3.825</westBoundingCoordinate>
          <eastBoundingCoordinate>3.825</eastBoundingCoordinate>
          <northBoundingCoordinate>51.345</northBoundingCoordinate>
          <southBoundingCoordinate>51.345</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/4803">
        <geographicDescription>DAS20_S09 &gt; station S09</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>4.07833333</westBoundingCoordinate>
          <eastBoundingCoordinate>4.07833333</eastBoundingCoordinate>
          <northBoundingCoordinate>51.37</northBoundingCoordinate>
          <southBoundingCoordinate>51.37</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/9105">
        <geographicDescription>DAS20_S01 &gt; station S01</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>3.57</westBoundingCoordinate>
          <eastBoundingCoordinate>3.57</eastBoundingCoordinate>
          <northBoundingCoordinate>51.41666667</northBoundingCoordinate>
          <southBoundingCoordinate>51.41666667</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/2585">
        <geographicDescription>DAS20_S22 &gt; station S22</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>4.39166667</westBoundingCoordinate>
          <eastBoundingCoordinate>4.39166667</eastBoundingCoordinate>
          <northBoundingCoordinate>51.21883333</northBoundingCoordinate>
          <southBoundingCoordinate>51.21883333</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <geographicCoverage id="http://marineregions.org/mrgid/9108">
        <geographicDescription>DAS20_S15 &gt; station S15</geographicDescription>
        <boundingCoordinates>
          <westBoundingCoordinate>4.27333333</westBoundingCoordinate>
          <eastBoundingCoordinate>4.27333333</eastBoundingCoordinate>
          <northBoundingCoordinate>51.31333333</northBoundingCoordinate>
          <southBoundingCoordinate>51.31333333</southBoundingCoordinate>
        </boundingCoordinates>
      </geographicCoverage>
      <temporalCoverage>
        <rangeOfDates>
          <beginDate>
            <calendarDate>2002-01-01</calendarDate>
          </beginDate>
          <endDate>
            <calendarDate>2006-01-01</calendarDate>
          </endDate>
        </rangeOfDates>
      </temporalCoverage>
      <taxonomicCoverage id="urn:lsid:marinespecies.org:taxname:107552">
        <generalTaxonomicCoverage>urn:lsid:marinespecies.org:taxname:107552</generalTaxonomicCoverage>
        <taxonomicClassification>
          <taxonRankName>species</taxonRankName>
          <taxonRankValue>Crangon crangon (Linnaeus, 1758)</taxonRankValue>
        </taxonomicClassification>
      </taxonomicCoverage>
      <taxonomicCoverage id="urn:lsid:marinespecies.org:taxname:120136">
        <generalTaxonomicCoverage>urn:lsid:marinespecies.org:taxname:120136</generalTaxonomicCoverage>
        <taxonomicClassification>
          <taxonRankName>species</taxonRankName>
          <taxonRankValue>Neomysis integer (Leach, 1814)</taxonRankValue>
        </taxonomicClassification>
      </taxonomicCoverage>
    </coverage>
    <methods>
      <methodStep>
        <description>
          <title>GCMS DDT analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS DDT sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS DDT biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS dieldrin biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS dieldrin sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS Dieldrin analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS HCB analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS HCB sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS HCB biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS lindane biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS lindane sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS Lindane analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS organotin analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De ionogene organotin-verbindingen komen via een schudextraktie m.b.v. methanol, azijnzuur en hexaan beschikbaar voor alkylering. De tri, di en mono-alkyltinverbindingen worden vervolgens geethyleerd m.b.v. NaB(Et)4. De tetra-alkyltin-verbindingen migreren door het toevoegen van (extra) water naar de hexaanfase. Na centrifugeren wordt de hexaanf</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS organotin sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS organotin biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PBDE biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PBDE sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PBDE analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PCB analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PCB sediment analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PCB biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PAK biotic analysis</title>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PAK analysis</title>
          <para>(1) Sampling protocol: Go Flo; opening and closing at certain depth, undisturbed watersample; Teflon coating evoiding contamination

(2) Lab protocol: De PAK’s worden geëxtraheerd uit het water door gebruik te maken van speeddisk C18 extractie filters (Baker). De filters worden eerst geconditioneerd met methanol en organisch vrij water. Daarna wordt het waterstaal over de filter gebracht met behulp van een vaccuümpomp. De filter wordt  nadien gedroogd, door nog enkele minuten de filter onder v</para>
        </description>
      </methodStep>
      <methodStep>
        <description>
          <title>GCMS PAK sediment analysis</title>
          <para>(1) Sampling protocol: Van Veen grijper staalnames

(2) Lab protocol: Een hoeveelheid sedimentmonster wordt verzeept met methanol en kaliumhydroxide gedurende 2 uur.Het mengsel wordt gefiltreerd en opgevangen in een scheitrechter waar het wordt geextraheerd met n-pentaan. Het extract wordt aan de rotovapor ingedampt en op een Al2O3 kolom gebracht. Laat nog 2 maal 1 ml eluent (1/1 volume n-pentaan en dichloromethaan) over de kolom lopen. Het eluent wordt tot 1 ml geconcentreerd met de rotovapor</para>
        </description>
      </methodStep>
      <sampling>
        <studyExtent>
          <description>
            <para>Entire sampling area</para>
          </description>
        </studyExtent>
        <samplingDescription>
          <para>GCMS DDT analysis; GCMS DDT sediment analysis; GCMS DDT biotic analysis; GCMS dieldrin biotic analysis; GCMS dieldrin sediment analysis; GCMS Dieldrin analysis; GCMS HCB analysis; GCMS HCB sediment analysis; GCMS HCB biotic analysis; GCMS lindane biotic analysis; GCMS lindane sediment analysis; GCMS Lindane analysis; GCMS organotin analysis; GCMS organotin sediment analysis; GCMS organotin biotic analysis; GCMS PBDE biotic analysis; GCMS PBDE sediment analysis; GCMS PBDE analysis; GCMS PCB analysis; GCMS PCB sediment analysis; GCMS PCB biotic analysis; GCMS PAK biotic analysis; GCMS PAK analysis; GCMS PAK sediment analysis</para>
        </samplingDescription>
      </sampling>
    </methods>
    <maintenance>
      <description>
        <para />
      </description>
      <maintenanceUpdateFrequency>unkown</maintenanceUpdateFrequency>
    </maintenance>
    <contact>
      <individualName>
        <givenName>Els</givenName>
        <surName>Monteyne</surName>
      </individualName>
      <organizationName>Koninklijk Belgisch Instituut voor Natuurwetenschappen; Departement Beheer van het Mariene Ecosysteem; Beheerseenheid Mathematisch Model Noordzee en Schelde-estuarium; Oostende</organizationName>
    </contact>
  </dataset>
  <additionalMetadata>
    <describes>
      <para>Extra metadata added by VLIZ to add parameter, platform info, as well as GBIF fields</para>
    </describes>
    <metadata>
      <parameter>DDT</parameter>
      <parameter>Dieldrin</parameter>
      <parameter>Hexachlorobenzene</parameter>
      <parameter>Lindane</parameter>
      <parameter>Organotin</parameter>
      <parameter>Polybrominated diphenyl ethers (PBDEs)</parameter>
      <parameter>Polychlorinated biphenyls (PCBs)</parameter>
      <parameter>Polycyclic aromatic hydrocarbons (PAHs)</parameter>
      <gbif>
        <dateStamp>2021-12-02T15:14:18+01:00</dateStamp>
        <hierarchyLevel>dataset</hierarchyLevel>
        <citation>Monteyne, E.; Roose, P.; Management Unit of the North Sea Mathematical Models - RBINS; (2003): Chemical analysis of PAK's, Organotin, PCB's, PBDE's and organochlorine pesticides as possible endocrine disruptors in Scheldt estuary.</citation>
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