Metadata Catalogue
Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary
This dataset results out of the study into ecotoxicological evaluation of the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies).
Default
Identification
- Publication Date
- 2021-12-02
- Title
-
Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary
- Abstract
-
This dataset results out of the study into ecotoxicological evaluation of the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies).
- Dataset Language
-
en
- Dataset Creator
- Metadata Provider
- Associated Party
- Dataset Contact
- Keywords (ASFA)
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Biomarkers
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Crustacea
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Energy
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Estrogens
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Metabolism
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Moulting
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Reproduction
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Vitellogenesis
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Geographic Coverage
- Geographic Description
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ANE, Scheldt Estuary
Geographic Coverage
- Geographic Description
-
EurOBIS calculated BBOX > station Bounding Box
Bounding Box
- West Bounding Coordinate
-
4.0780000686645
- East Bounding Coordinate
-
4.2249999046326
- North Bounding Coordinate
-
51.369998931885
- South Bounding Coordinate
-
51.365001678467
Geographic Coverage
- Geographic Description
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Westerschelde
Geographic Coverage
- Geographic Description
-
Zeeschelde
Geographic Coverage
- Geographic Description
-
DAS56_S12 > station S12
Bounding Box
- West Bounding Coordinate
-
4.225
- East Bounding Coordinate
-
4.225
- North Bounding Coordinate
-
51.365
- South Bounding Coordinate
-
51.365
Geographic Coverage
- Geographic Description
-
DAS56_S07 > station S07
Bounding Box
- West Bounding Coordinate
-
4
- East Bounding Coordinate
-
4
- North Bounding Coordinate
-
51.43666667
- South Bounding Coordinate
-
51.43666667
Geographic Coverage
- Geographic Description
-
DAS56_S04 > station S04
Bounding Box
- West Bounding Coordinate
-
3.825
- East Bounding Coordinate
-
3.825
- North Bounding Coordinate
-
51.345
- South Bounding Coordinate
-
51.345
Geographic Coverage
- Geographic Description
-
DAS56_S09 > station S09
Bounding Box
- West Bounding Coordinate
-
4.07833333
- East Bounding Coordinate
-
4.07833333
- North Bounding Coordinate
-
51.37
- South Bounding Coordinate
-
51.37
Geographic Coverage
- Geographic Description
-
DAS56_S01 > station S01
Bounding Box
- West Bounding Coordinate
-
3.57
- East Bounding Coordinate
-
3.57
- North Bounding Coordinate
-
51.41666667
- South Bounding Coordinate
-
51.41666667
Geographic Coverage
- Geographic Description
-
DAS56_S22 > station S22
Bounding Box
- West Bounding Coordinate
-
4.39166667
- East Bounding Coordinate
-
4.39166667
- North Bounding Coordinate
-
51.21883333
- South Bounding Coordinate
-
51.21883333
Geographic Coverage
- Geographic Description
-
DAS56_S15 > station S15
Bounding Box
- West Bounding Coordinate
-
4.27333333
- East Bounding Coordinate
-
4.27333333
- North Bounding Coordinate
-
51.31333333
- South Bounding Coordinate
-
51.31333333
Temporal Coverage
Range of Dates
- Begin Date
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2002-12-04
- End Date
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2002-12-06
Temporal Coverage
Range of Dates
- Begin Date
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2003-03-17
- End Date
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2003-03-21
Temporal Coverage
Range of Dates
- Begin Date
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2003-06-30
- End Date
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2003-07-04
Temporal Coverage
Range of Dates
- Begin Date
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2004-02-04
- End Date
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2004-02-05
Taxonomic Coverage
- General Taxonomic Coverage
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urn:lsid:marinespecies.org:taxname:120136
Taxonomic Classification
- Taxonomic Rank Name
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species
- Taxonomic Rank Value
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Neomysis integer (Leach, 1814)
License Information
- Intellectual Rights
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This work is licensed under a
Creative Commons Attribution (CC-BY) 4.0 License
.
Additional Metadata
- describes
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Extra metadata added by VLIZ to add parameter, platform info, as well as GBIF fields
Metadata
- parameter
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Growth factor
- parameter
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Growth rate
- parameter
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Mysid energy metabolism
- parameter
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Mysid intermoult period
- parameter
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Mysid testosteron metabolism
- parameter
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Mysid vitelline concentration
GBIF Metadata Block
- Date Stamp
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2021-12-02T15:30:28+01:00
- Hierarchy Level
-
dataset
- Citation
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Ghekiere A., Verslycke T., De Smet L., Van Beeumen J., Janssen C.R.; Environmental toxicology research group - Ugent; (2004): Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary
Physical
- Object Name
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Online dataset
- Character Encoding
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UTF-8
Data Format
Externally Defined Format
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HTML
Distribution
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Online dataset
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UTF-8
Data Format
Externally Defined Format
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HTML
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- Object Name
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Archived file
- Character Encoding
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UTF-8
Data Format
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Archived file
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UTF-8
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Archived file
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UTF-8
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Online dataset
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UTF-8
Data Format
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HTML
Distribution
Physical
- Object Name
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Dataset dedicated website
- Character Encoding
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UTF-8
Data Format
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- Format Name
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HTML
Distribution
Online
Physical
- Object Name
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Online dataset
- Character Encoding
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UTF-8
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HTML
Distribution
Physical
- Object Name
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Online dataset
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UTF-8
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Metadata
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UTF-8
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Distribution
Online
Methods
• Method
Method Step
- Description
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and the increase in length at each molting event (the growth factor, GF in % of the pre-molt length)
Method Step
- Description
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length incresase (growth rate, µm/day) during IMPs
Method Step
- Description
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The energy allocation of the estuarine mysid Neomysis integer were investigated using the cellular energy allocation assay (CEA), energy reserves (protein, lipid and sugar) and energy consumption (as derived from the cellular respiration rate) were integrated into a general indicator of physiological stress.
Adult Neomysis integer specimens were sorted out on board, sex was not taken into account and no selection was made to avoid gravid females. The collected mysids were shock-frozen in liqui
Method Step
- Description
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time (days) between two successive molts (intermolt period; IMP)
Method Step
- Description
-
In vivo exposure to testosterone:
Juvenile Neomysis integer were taken from the different samplingsites during the campaigns of March and June and individually placed into 5 ml glass recipients containing 2 ml water (5 PSU). Two micrograms of testosterone dissolved in 10 µl methanol was added to the recipient and a 6 h exposure period was applied.
Extraction and LC-MSn analysis of testosterone metabolites and endogenous steroids:
N. integer were isolated from the exposure medium, shock-
Method Step
- Description
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-Vitellin purification (gel filtration)
-polyclonal anitbody production against vitellin
-development of ELISA protocol
Sampling
- Study Extent
-
Entire sampling area
- Sampling Description
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Moulting measuring protocol growth factor; Moulting measuring protocol growth rate; Cellular Energy Allocation; Moulting measuring protocol intermoult period; LCMS testosteron; Enzyme-linked immunosorbent assay (ELISA)