The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

The dataset contains 412 records of Diptera species from the families Limoniidae, Pediciidae and Tipulidae, collected between 2003 to 2019 in Portugal, including the Azores and Madeira archipelagos (Ferreira et al., 2021; Oosterbroek et al., 2020; Starý, 2014). Specimens have been identified to species or subspecies level, in a total of 83 species representing 58% of the Craneflies known for Portugal. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and the majority was stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by the EnvMetaGen and PORBIOTA projects. The dataset “DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae” is part of a group of Diptera datasets published by IBI, the first of which, “The InBIO Barcoding Initiative Database: Diptera 01” (https://doi.org/10.15468/q1bvt3) has already been made available through GBIF. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

The dataset contains 219 entries with 241 records of specimens from the families Limoniidae, Pediciidae and Tipulidae, collected between 1985 to 2019 in continental Portugal (Oosterbroek et al., 2020, part). All specimens have been identified to species level, in a total of 65 species representing 45% of the craneflies known for continental Portugal. Specimens were collected in 67 different localities. The dataset “Diptera 03 - Further records on Portuguese Limoniidae, Pediciidae and Tipulidae” is part of a group of DNA barcoding datasets of Diptera conducted within the InBIO Barcoding Initiative (IBI), two of which have already been made available through GBIF (“The InBIO Barcoding Initiative Database: Diptera 01”, https://doi.org/10.15468/q1bvt3; “The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae”, https://doi.org/10.15468/f6256w). IBI is funded by the EnvMetaGen and PORBIOTA projects. Most specimens (142) are deposited in the IBI collection. Other specimens are deposited in the Collection Rui Andrade (Porto, Portugal, 76 specimens), the Hunterian Zoology Museum (Glasgow, U.K., 22 specimens) and in the Naturalis Biodiversity Center, Zoology collections (Leiden, Netherlands, 1 specimen).