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This service represents the Step 2 of the Metabarcoding Workflow within the Internal Joint Initiative. It aims at converting the CSV into rdata. It takes as input the Species_occ.csv file (output of the Step 1 Metabarcoding Runner), verifies the checklists available for each country and retrieves the first one. It produces two rdata files, that will be the inputs for the Step 3 GBIF NIS Verifier of the Metabarcoding workflow.
This service aims to compute a list of taxa IDs detected from metabarcoding sequences of environmental DNA (eDNA) samples. This service contains eight substeps (1.1 to 1.8) implemented as a unique step. The eight substeps perform (1) sequencing error correction (using BayesHammer-SPAdes); (2) pairwise alignment, (3) pre-filtering, (4) dereplication, (5) attribute filtering, (6) clustering and OTU tab-producer (using OBITools); (7) taxonomic assignment (using blastn); (8) OTUs table generator. Several types of eDNA samples can be processed (i.e. water, feces, soil). It represents the Step 1 of the Metabarcoding Workflow within the Internal Joint Initiative.