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    The dataset contains one record of the species Wesmaelius subnebulosus (Stephens, 1836) (Neuroptera, Hemerobiidae), a brown lacewing species, collected in continental Portugal in 2017, and first recorded for the country in Oliveira and Ferreira (2020). The specimen was detected and captured using a light-trap setup. A tissue sample, one leg, was removed to be used for DNA extraction. Sequencing of the 658 bp COI DNA barcode was conducted within the IBI project and the DNA sequence was submitted to BOLD (Barcode of Life Data System) database. The preserved specimen and DNA extract are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).