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    The dataset contains records of freshwater fishes collected in the Tua river sub-basin and included in the Complementary Studies to the Environmental Impact Assessment of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected in August 2011, during field visits to 17 fixed stations. Sampling was made using electrofishing, and it targeted all fish species. The following species were recorded: Achondrostoma oligolepis, Cobitis calderoni, Gambusia holbrooki, Lampetra planeri, Lepomis gibbosus, Luciobarbus bocagei, Pseudochondrostoma duriense, Salmo trutta subsp. fario, Squalius alburnoides, Squalius carolitertii.