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    The dataset contains records of Phytobenthos (Diatoms) collected in the watershed of the River Tua and its tributaries, and included in the Ecological Monitoring Program (2011-2017) of the Environmental Impact Assessment of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected in the spring 2011-2015 during field visits to 17 sampling points distributed along the valley of river Tua (Northeast Portugal) and its tributaries, up to the intersection with rivers Rabaçal and Tuela, 52 kms upstream. The Ecological Monitoring Program (2011-2015) aims to evaluate the impacts of dam construction in the water quality of the river, before and during the construction process. The occurrence of the target taxa was documented through 33526 records of species presence and number of individuals (valves).