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The dataset contains records of reptiles (Reptilia) collected in the lower valley of the River Tua, and included in the Ecological Monitoring Program (2011-2017) of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected between July 2011 and August 2017 during field visits to 24 (2011-2014) or 28 (2014-2017) sampling transects with favorable habitats, in 24 2x2 km UTM squares in the area of the lower Tua valley directly or indirectly affected by the Foz Tua dam. The presence of individuals was recorded by direct observation.