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    The dataset contains 234 records of Lacewings (Neuroptera) species collected from 2006 to 2019 in continental Portugal (Oliveira et al., 2021). Specimens were detected and captured by direct search of the environment and by using both UV and mercury-vapor lamps to attract the insects. Captured specimens were preserved in 96% ethanol. All captured specimens were identified to species level. Samples of each species were selected for DNA sequencing based on their geographic provenance. From each specimen, one leg (tissue sample) was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).