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    The dataset contains 11 records of Stag beetle (Lucanidae) species collected from 2015 to 2019 in continental Portugal. All specimens were identified to species level, and belong to 3 species of the Lucanidae (Coleoptera). Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (a leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences available were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).